间歇性加载周期性压力增加骨细胞分泌一氧化氮和前列腺素E_2  

Nitric Oxide and Prostaglandin E_2 Secretion in Osteocytes Induced by Intermittent Cyclic Compressive Force

作　　者：阴健[1,2] 浩志超[1,2] 廖爽[1,2] 刘颖[1,2] 沈颉飞[1,2] 廖运茂[1] 王航[1,2] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西口腔医学院修复科,成都610041

出　　处：《生物医学工程学杂志》2014年第3期619-624,共6页Journal of Biomedical Engineering

基　　金：四川省科技厅科技支撑项目资助(2011SZ0157);国家自然基金资助项目(81170941);四川大学国家级大学生创新创业训练计划资助项目(201210610097)

摘　　要：为探讨间歇性加载力学刺激对骨细胞机械敏感性的影响,应用我们实验室自制的细胞加力装置对MLOY4骨样细胞施加周期性压力(CCF),观察不同时长间歇期(5、15和30s)的力学刺激对骨细胞机械敏感性的影响。本研究运用Griess法及酶联免疫吸附测定(ELISA)法分别检测一氧化氮(NO)和前列腺素E2(PGE2)分泌水平,采用免疫荧光对细胞骨架F-actin进行染色观察。结果发现:15s以上间歇期压力加载组骨细胞分泌NO及PGE2明显高于持续加载组(P<0.05),并且间歇性加载压力可提高细胞骨架顺应力学刺激方向的定向排列。该结果提示,间歇性加载周期性压力可显著提高骨细胞的机械敏感性,且与细胞骨架的定向排列有一定关系。This paper is aimed to investigate the effect of rest-inserted loading on the mechanosensitivity of osteo- cytes. In the investigation, cultured MLO-Y4 osteocyte-like cells were strained on cyclic compressive force （CCF） by the self-made compressive loading device. Then we observed the effect of different rest periods-inserted loading （5 s, 15 s, 30 s, respectively） on the mechanosensitivity of osteocytes. We then determined the levels of secreted nitric oxide （NO） and prostaglandin E2 （PGE2） by Griess method and enzyme linked immunosorbent assay （ELISA）, re- spectively. We then stained the cytoskeleton F-actin using immunofluorescence. We found that the expressions of NO and PGE2 in rest-inserted strained groups （〉15 s） were significantly increased compared to those in the continu- ous strained group. And rest-inserted loading promoted the parallel alignment of stress fibers. It indicates that rest- inserted loading could promote the mechanosensitivity of osteocytes, and this might be related to the parallel align- ment of stress fibers.

关 键 词：力学刺激 间歇期 骨细胞 机械敏感性 

分 类 号：R318.0[医药卫生—生物医学工程] R34[医药卫生—基础医学]