活化沉默信息调节因子1对大鼠严重烫伤早期心肌损害的影响  

Effects of activating silent information regulator 1 on early myocardial damage in severely burnedrats

作　　者：樊磊[1] 白晓智[1] 杨龙龙[1] 王姝月[1] 杨晨[1] 李超[1] 苏琳琳[1] 吕根法 胡大海[1] 

机构地区：[1]第四军医大学西京医院全军烧伤中心、烧伤与皮肤外科,西安710032 [2]解放军第五三四医院外三科

出　　处：《中华烧伤杂志》2014年第3期194-198,共5页Chinese Journal of Burns

摘　　要：目的 探讨活化沉默信息调节因子1（SIRT1）对大鼠严重烫伤早期心肌损害的作用.方法 取24只健康雄性SD大鼠,按照随机数字表法分为假伤组、单纯烫伤组、白藜芦醇治疗组,每组8只.单纯烫伤组和白藜芦醇治疗组大鼠背部于95℃热水浴18s,制成30％ TBSAⅢ度烫伤模型,2组大鼠即刻分别腹腔注射生理盐水10 mL（50 mL/kg）与相同剂量生理盐水＋白藜芦醇10 μL（1 g/mL,50 mg/kg）;假伤组大鼠仅背部于20℃室温水浴18s模拟烫伤过程.伤后6h收集3组大鼠心脏组织及腹主动脉血,HE染色观察组织形态,ELISA法检测血清肌酸激酶（CK）、乳酸脱氢酶（LDH）含量,蛋白质印迹法和实时荧光定量RT-PCR法检测组织中SIRT1、半胱氨酸天冬氨酸蛋白酶3（caspase-3）的蛋白和SIRT1、caspase-3、IL-1β、TNF-α mRNA表达水平（蛋白水平以灰度值表示）.对数据行单因素方差分析、LSD-t检验. 结果 （1）假伤组大鼠心肌纤维呈不规则圆柱形、排列整齐、横纹清晰.单纯烫伤组大鼠心肌间质水肿,心肌纤维排列紊乱并断裂、溶解.白藜芦醇治疗组大鼠心肌间质水肿、心肌纤维排列紊乱及溶解程度均较单纯烫伤组减轻.（2）单纯烫伤组大鼠血清CK、LDH含量分别为（2 385 ±712）、（2 551±196）U/L,显著高于假伤组的（290±59）、（759±60） U/L（￡值分别为9.466、25.452,P值均小于0.01）.白藜芦醇治疗组大鼠血清CK、LDH含量[（1 336±149）、（2 209±133）U/L]显著低于单纯烫伤组（￡值分别为-4.506、-4.860,P值均小于0.01）.（3）单纯烫伤组大鼠心脏组织SIRT1、caspase-3蛋白表达量分别为0.47±0.11、0.48 ±0.12,显著高于假伤组的0.18±0.06、0.09 ±0.05（t值分别为4.813、9.014,P值均小于0.01）.白藜芦醇治疗组大鼠心脏组织SIRT1蛋白表达量（0.74±0.18）显著高于单纯烫伤组（t=4.561,P＜0.01）,caspase-3蛋白表达量（0.21 ±0.08）显著低于单纯烫伤组（￡=-6.239,PObjective To explore the effects of activating silent information regulator 1 （SIRT1） on early myocardial damage in severely burned rats.Methods Twenty-four healthy male SD rats were divided into sham injury group （SI）,scald group （S）,and resveratrol （RSV） treatment group （RT） according to the random number table,with 8 rats in each group.Rats in groups S and RT were inflicted with 30% TBSA full-thickness scald on the back by immersing in 95 ℃ water for 18 s.Immediately after injury,rats in group S were intraperitoneally injected with 10 mL normal saline （50 mL/kg） and those in group RT with 10 mL normal saline （50 mL/kg） ＋ 10 μL RSV in the concentration of 1 g/mL （50 mg/kg）.Backs of rats in group SI were immersed in 20 ℃ room temperature water for 18 s to simulate the scald process.Heart tissues and aorta abdominalis blood samples were collected at post injury hour （PIH） 6.The histomorphology of heart tissues was observed with HE staining.The serum contents of creatine kinase （CK） and lactate dehydrogenase （LDH） were determined with ELISA.The protein expressions of SIRT1 and caspase-3 and mRNA expressions of SIRT1,caspase-3,IL-1β,and TNF-α in heart tissue specimens were determined with Western blotting and real-time fluorescent quantitative RT-PCR （with protein level denoted as gray value）.Data were processed with one-way analysis of variance and LSD-t test.Results （1） In group SI,myocardial fibers were in irregularly cylindrical shape,neatly arranged,and the transverse striation were distinct.In group S,myocardial interstitial edema,disorder of myocardial fiber arrangement,and cytoplasm destruction were observed.In group RT,the degrees of myocardial interstitial edema,disorder of myocardial fiber arrangement,and cytoplasm destruction were alleviated in comparison with those of group S.（2） The serum contents of CK and LDH of rats in group S were respectively （2 385 ±712） and （2 551 ± 196） U/L,which were significantly higher than those in

关 键 词：烧伤 心肌 半胱氨酸天冬氨酸蛋白酶3 白细胞介素1Β 肿瘤坏因子α 沉默信息调节因子1 

分 类 号：R644[医药卫生—外科学]