尖吻蝮蛇毒抑瘤组分I对人肺癌A549细胞增殖抑制作用  

Inhibitory effect of anti-tumor componentⅠfrom Agkistrodon acutus venom on human lung cancer A549 cells

作　　者：徐平[1] 张根葆[1,2] 王斐[1] 吴娟[1] 皇甫政彤[1] 

机构地区：[1]皖南医学院病理生理教研室 [2]皖南医学院蛇毒研究所

出　　处：《中国临床药理学与治疗学》2014年第5期493-496,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究尖吻蝮蛇毒抑瘤组分I(AAVC-I)对人肺癌A549细胞增殖抑制及凋亡的作用。方法:采用MTT法检测不同浓度的AAVC-I对A549细胞的24h与48h抑制率;应用HE染色、Hoechst33258荧光染色,从形态学观察细胞凋亡;采用免疫组化的方法,检测bax蛋白表达的变化。结果:MTT显示,AAVC-I能抑制A549细胞增殖,呈时间和剂量依赖性;AAVCI处理24h后,镜下可见细胞核固缩,核深染及凋亡小体;免疫组化显示,随药物浓度增加,平均光密度值呈递增趋势,表明bax蛋白表达相应上调。结论:尖吻蝮蛇毒抑瘤组分I对人肺癌A549细胞具有抑制作用,并诱导其凋亡,可能与bax表达上调有关。AIM： To research the inhibitory and apoptosis effect of anti-tumor component I （AAVC-I） from Agkistrodon acutus venom on human lung cancer A549 cells. METHODS： The inhibition rate after 24 h and 48 h with different concentrations of AAVC-I on A549 was detected by MTT method. The form of apoptosis was observed by HE staining and Hoechst fluorescent staining. The expression change of bax protein after AAVC-I treatment was detected by immunohistochemical staining method. RESULTS： MTT showed that AAVC-I could inhibit the proliferation of A549 in a time- and dose-dependent manner. The form of apoptosis in A549 could be observed by ordinary microscope and fluorescent staining when treated with AAVC-I for 24 h. Immunohistochemical staining showed that the mean light density had a trend of increase in dose dependency, which indicated that the expression of bax increased accordingly. CONCLUSION： AAVC-I had an inhibitory effect on A549 cell proliferation, apoptosis promoting effect as well, which maybe related to the increase of bax expression.

关 键 词：尖吻蝮蛇毒抑瘤组分I A549 细胞凋亡 BAX 

分 类 号：R965.2[医药卫生—药理学]