大鼠胃浆膜下间隙胰岛移植的存活率及其功能的实验研究  

Experimental study of survival rate and function of stomach subserous transplanted islets in inbred rats

作　　者：刘新农[1] 胡三元[1] 张光永[1] 韩海峰[1] 胡春晓[1] 王磊[1] 

机构地区：[1]山东大学齐鲁医院普外科,山东济南250012

出　　处：《山东大学学报（医学版）》2014年第5期49-52,62,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展计划(2012GSF11850)

摘　　要：目的探讨大鼠胃浆膜下间隙胰岛移植的可行性及其移植效果。方法应用Lewis近交系雄性大鼠,采用链脲菌素诱导糖尿病模型;同种系大鼠体外胰岛分离、纯化,将一定数量的胰岛植入糖尿病大鼠胃浆膜下间隙(胃组),同期将同等数量的胰岛植入肾被膜下间隙(肾组)作为对照,评估移植后糖尿病大鼠的血糖水平、腹腔葡萄糖耐量试验及胰岛移植物存活率。结果胰岛移植术后前3周,胃组和肾组内糖尿病大鼠的血糖水平较移植前均明显下降(P<0.05),两组大鼠间血糖水平无差异、糖耐受功能良好(P>0.05);两移植部位的胰岛被免疫组织化学染色有效确认,移植胰岛周围有少量炎性细胞浸润,肾组织样本内胰岛周围的炎细胞浸润更明显。肾组和胃组糖尿病大鼠血糖水平分别于胰岛移植术后第5、4周开始升高。术后第6周两组大鼠血糖水平均升高且糖耐量减低,免疫组织化学染色检查显示,两移植部位内仅残余散在的胰岛细胞。结论胃浆膜下间隙胰岛移植的短期效果与肾被膜下胰岛移植相同,但其血糖控制效果比肾被膜下胰岛移植更佳,而其长期效果有待进一步证实。Objective To explore the feasibility of transplanting islets into the stomach subserous space in diabetic inbred rats. Methods Lewis inbred male rats were enrolled as islets donors and recipients. Diabetes was induced by the administration of streptozotocin. Equal numbers of fresh islets were transplanted into the stomach subserous space and into the renal subcapsular space,respectively. The glucose level,intraperitoneal glucose tolerance and survival rate of islets transplanted were detected afterwards. Results The glucose level of all diabetic rats receiving islets in stomach subserous or renal subcapsular space significantly decreased 3 weeks after transplantation（P ＆lt; 0. 05）. All rats had good glucose tolerance,and there was no significant difference in the glucose level between the two groups（P ＆gt; 0. 05）. The islets transplanted into those two sites were identified by immunohistochemical detection using anti-insulin antibody, with a fewof inflammatory cells infiltrating around the islets. The infiltration was more obvious around islets located in the renal subcapsular space. The glucose level of rats with islets transplanting into renal subcapsular space and stomach subserous space gradually increased again at the 5th and 4th weeks after transplantation,respectively. The glucose level of all the rats in the two groups significant increased with a reduced glucose tolerance and the immunohistochemical detection showed that there were only dispersed islets left in the two sites at the 6th week after transplantation. Conclusion Islet transplantation into stomach subserous space is technically feasible and owns a better effect in improving theglucose of diabetic rats than that into renal subcapsular space in the early state. However,the long-term effects remain to be confirmed by other studies.

关 键 词：胰岛移植 胃浆膜下间隙 肾被膜下间隙 大鼠 近交Lew 

分 类 号：R587.1[医药卫生—内分泌]