孕期吸烟对SD大鼠胚胎心脏发育的影响  

Effect of maternal smoking on embryonic heart development in SD rats

作　　者：叶力通[1,2] 江雪燕[1,2] 张梦珍[2] 潘宇[2] 林秋雄[2] 单志新[2] 姜霖[2] 陈瑶[2] 余细勇[2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广东省人民医院(广东省医学科学院),广东省心血管病研究所,广东广州510080

出　　处：《热带医学杂志》2014年第5期551-554,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(81120108003;81330007);国家973计划(2012CB526600);广州市珠江科技新星专项(2011J2200049)

摘　　要：目的探讨孕期吸烟对SD大鼠胚胎心脏发育的影响及分子机制。方法将24只怀孕SD母鼠随机分为4组:正常对照组、10支烟/d组、20支烟/d组、40支烟/d组,每组6只,并于孕期0.5 d(GD 0.5)后开始给予吸烟处理直至分娩。采用超声多普勒Vevo2000检测出生后1 d的新生乳鼠的左心结构及其心脏功能;采用Color-Doppler检测心室血流方向,以确认室间隔的发育是否异常。心功能检测完毕后,收集各组新生乳鼠心脏组织,采用Western-blot检测心肌特异转录因子GATA4的表达量。结果各组新生乳鼠心脏重量无明显差异,40支烟/d处理组乳鼠体重显著下降,与对照组相比,差异有统计学意义(P<0.001)。孕期持续给予40支烟/d被动吸烟处理能显著降低左心室重量、左心室舒张/收缩末期前壁厚度和左心室舒张/收缩末期后壁厚度,差异均有统计学意义(P<0.05)。孕期给予20支烟/d和40支烟/d处理后,新生乳鼠的GATA4蛋白表达呈剂量依赖性下调,40支烟/d组与对照组相比,差异有统计学意义(P<0.05)。结论母鼠在怀孕期间接受被动吸烟后,引发后代新生乳鼠的心功能受损,其机制可能与下调心肌特异转录因子GATA4表达相关。Objective To investigate the effect of maternal smoking on cardiac development in Sprague Dawley rats and the underlying mechanism. Methods Pregnant Sprague Dawley rats （SD rats） were randomly dived into 4 groups： Control, 10 cigarettes/day, 20 cigarettes/day, 40 cigarettes/day, and exposed to passive cigarettes smoking throughout gestation. On postnatal day 1 （PND1）, echocardiography was performed to assess the left ventricular structure and cardiac function in neonatal rats. Color Doppler was used to detect the blood direction and velocity and confirm the presence of ventricular septum. Neonatal heart tissues from each group were collected after echocardiography assessment, and western blot was used to detect the expression level of cardiac transcriptional factor GATA4. Results （1） The average of heart weight had no significant change between each group, however the body weight decreased significantly in 40 cigarettes/day group （P〈0.001）. （2） Maternal smoking to 40 cigarettes daily significantly reduced the weight of left ventricular （LV）, LV diastolic/systolic anterior and LV diastolic/systolic posterior wall （P〈0.05）. （3） Matemal smoking to 40 cigarettes/day significantly down-regulated the expression of GATA4 （P〈0.05）. Conclusion Maternal smoking induced cardiac malfunction in rat offspring, which might correlate with down regulation of GATA4 expression.

关 键 词：孕期吸烟 SD新生乳鼠 心脏发育 心肌转录因子 GATA4 

分 类 号：Q322.11[生物学—遗传学]