不同接种方式对组织工程牙龈种子细胞黏附效果的影响  

Effects of different implant methods on in vitro adhesion of tissue-engineered gingival cells

作　　者：钟代琴[1,2,3] 周勤虎[4] 靳趁心[3] 梁妍[3] 

机构地区：[1]口腔疾病与生物医学重庆市重点实验室,重庆市400015 [2]重庆医科大学附属口腔医院,重庆市400015 [3]天津医科大学附属口腔医院,天津市300017 [4]重庆市公安局刑警总队,重庆市400021

出　　处：《中国组织工程研究》2014年第21期3299-3303,共5页Chinese Journal of Tissue Engineering Research

基　　金：天津市高等学校科技发展基金项目(20110140)~~

摘　　要：背景:如何在体外将支架材料和种子细胞高效地复合以构建组织工程牙周组织是目前牙周病治疗及牙周缺损修复研究的重要方向。目的:比较传统沉淀接种法和胶原包裹接种法的细胞黏附状况,优化细胞接种方式。方法:将一定浓度的犬牙龈成纤维细胞,分别采用传统沉淀接种法和胶原包裹接种法接种到聚乳酸-壳聚糖-明胶梯度孔径和均匀孔径支架上,通过细胞计数测定支架上贴附的细胞数量,计算其接种率,并进行对比分析。结果与结论:采用胶原凝胶包裹接种法将细胞接种至均匀孔径支架和梯度孔径支架上,其接种率均明显高于传统的沉淀接种法(P<0.01)。用胶原凝胶包裹种子细胞行细胞接种可以有效提高种子细胞的接种率,增加支架上的细胞初始浓度,可以选用胶原凝胶包裹细胞接种方式用于牙龈工程组织构建。BACKGROUND：How to construct tissue-engineered periodontal tissue through efficient combination of scaffold materials and seed cells in vitro is an important direction of current research. OBJECTIVE：To compare the cellseeding efficiency of traditional static seeding method and col agen enclose seeding method to select a more appropriate seeding method for cellattach. METHODS：Gingival fibroblasts from dogs at certain concentrations were seeded onto polylactic acid-chitosan-gelatin scaffolds with gradient and uniform pore sizes using the traditional static seeding method and col agen enclose seeding method, respectively. The number of adherent cells was counted to calculate the seeding efficiency for data comparison. RESULTS AND CONCLUSION：The cellseeding efficiency of the col agen enclose seeding method was higher than that of the traditional static seeding method （P〈0.01）. The col agen enclose seeding method can significantly improve the seeding efficiency and increase cellinitial concentration on the scaffold, and this method can be used for gingival tissue engineering.

关 键 词：生物材料 口腔生物材料 口腔组织工程 种子细胞 沉淀接种法 胶原包裹接种法 聚乳酸-壳聚糖-明胶 细胞接种率 牙龈成纤维细胞 

分 类 号：R318[医药卫生—生物医学工程]