新型自组装水凝胶NBD/RADA16可促进前成骨细胞的分化  

Preosteoblastic differentiation of MC3T3 E1 cells on the new-type self-assembling peptide hydrogel NBD/RADA16

作　　者：赵刚[1] 李京玲[1] 王丹[1] 于静[1] 赵艳宇[1] 满大鹏[1] 

机构地区：[1]佳木斯大学第二附属医院口腔医学院,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2014年第21期3329-3333,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:RADA16是较成熟的自组装纳米短肽材料,在亲水面往复形成互补离子键,可组装为纳米纤维,并且能够促MC3T3 E1细胞的黏附、伸展和增殖。目的:观察新型自组装多肽水凝胶NBD/RADA16对小鼠前成骨细胞MC3T3 E1成骨分化能力的影响。方法:将MC3T3 E1细胞分别接种于自组装多肽水凝胶NBD/RADA16与RADA16水凝胶中,进行成骨诱导培养,以单纯成骨诱导培养的细胞为对照。诱导培养1,3,6 d检测细胞碱性磷酸酶活性;诱导培养7 d后,Western Blot检测细胞骨形态发生蛋白2的表达;诱导培养21 d后,茜素红染色观察细胞钙化结节。结果与结论:MC3T3 E1细胞在NBD/RADA16多肽水凝胶上生长状态良好,优于在RADA16上生长的细胞。自组装多肽水凝胶NBD/RADA16上MC3T3 E1细胞的碱性磷酸酶活性高于RADA16水凝胶上及单纯成骨诱导培养的细胞(P<0.01)。自组装多肽水凝胶NBD/RADA16上MC3T3 E1细胞的矿化基质沉积、骨形态发生蛋白2表达高于RADA16水凝胶上的细胞(P<0.01)。结果提示NBD/RADA16自组装多肽水凝胶较RADA16水凝胶更能促进MC3T3 E1细胞的成骨分化。BACKGROUND：RADA16 is a mature amphiphilic self-assembling peptide, which can be assembled into nanofibers, and promote MC3T3 E1 cellattachment, spreading and proliferation. OBJECTIVE：To observe the effects of the new-type self-assembling peptide hydrogel NBD/RADA16 on osteogenic differentiation of mouse preosteoblasts MC3T3 E1. METHODS：The MC3T3 E1 cells were inoculated in NBD/RADA16 self-assembling peptide hydrogel and RADA16 hydrogel for osteogenic induction. cells undergoing simple osteogenic induction served as controls. After culture for 1, 3, 6 days, the activity of alkaline phosphatase was detected. After 7 days, western blot assay was used to determine the expression of bone morphogenetic protein-2. After 21 days, alizarin red staining was used to observe calcified nodules. RESULTS AND CONCLUSION：MC3T3 E1 cells grew wel on the NBD/RADA16 peptide hydrogel, which were superior to those on the RADA16 hydrogel. The activity of alkaline phoshpatase was higher in the NBD/RADA16 group than the RADA16 and control groups （P〈0.01）. Compared with the RADA16 hydrogel, mineralized matrix deposition and expression of bone morphogenetic protein-2 were higher on the NBD/RADA16 peptide hydrogel （P〈0.01）. These findings indicate that the NBD/RADA16 peptide hydrogel is superior to the RADA16 hydrogel for promoting the osteogenic differentiation of MC3T3 E1 cells.

关 键 词：生物材料 纳米材料 NBD RADA16 多肽水凝胶 三维培养 成骨细胞分化 

分 类 号：R318[医药卫生—生物医学工程]