产前应用地塞米松和盐酸氨溴索对早产鼠肺组织表面活性蛋白-B mRNA和甲状腺转录因子-1表达的影响  

Effects of antenatal dexamethasone and ambroxol on expression of surfactant protein-B mRNA and ;thyroid transcription factor-1 in pulmonary tissue of premature rats

作　　者：江莲[1] 李凡[2] 张文婷[1] 张会芬[1] 李梅[1] 刘翠萍[1] 

机构地区：[1]河北医科大学第四医院儿科,石家庄050011 [2]郑州大学第三附属医院儿科

出　　处：《中华围产医学杂志》2014年第6期396-402,共7页Chinese Journal of Perinatal Medicine

摘　　要：目的：探讨地塞米松与盐酸氨溴索对早产鼠肺组织中表面活性蛋白（surfactant protein，SP）-B mRNA及甲状腺转录因子（thyroid transcription factor，TTF）-1表达的影响及其作用机制。方法采用清洁级Sprague-Dawley大鼠，16只孕鼠随机分为4组，每组4只：地塞米松2剂组（分别于妊娠第17和18天肌内注射地塞米松0.2 mg/kg）、地塞米松1剂组（妊娠第18天肌内注射地塞米松0.2 mg/kg）、盐酸氨溴索组（分别于妊娠第16、17和18天腹腔注射盐酸氨溴索100 mg/kg）、对照组（分别于妊娠第16、17和18天腹腔注射生理盐水）。4组孕鼠均于妊娠第19天剖宫产取出胎鼠制成早产鼠模型，每组早产鼠中随机选择20只作为研究对象。计算肺重与体重的比值，光镜下观察肺形态发育，并计算肺泡腔表面积与肺泡间隔表面积的比值。采用免疫组织化学检测TTF-1蛋白表达。采用逆转录聚合酶链反应技术检测SP-B mRNA表达。统计学方法采用单因素方差分析、Student-Newman-Keuls法、Pearson积差相关分析。结果（1）地塞米松2剂组、地塞米松1剂组、盐酸氨溴索组和对照组肺重与体重的比值分别为（6.5±0.6）、（7.9±0.8）、（9.5±0.8）与（9.5±0.9） mg/g，4组间比较差异有统计学意义（F=67.69，P〈0.01）；地塞米松2剂组和1剂组均低于对照组（q值分别为17.143和9.143，P值均〈0.01）及盐酸氨溴索组（q值分别为17.143和9.143，P值均〈0.01）；地塞米松2剂组低于1剂组（q=8.000，P〈0.01）。（2）地塞米松2剂组、地塞米松1剂组、盐酸氨溴索组和对照组肺泡腔表面积与肺泡间隔表面积的比值分别为2.19±0.15、1.70±0.18、1.67±0.13与1.08±0.12，4组间比较差异有统计学意义（F=190.85，P〈0.01）；地塞米松2剂组、地塞米松1剂组和盐酸氨溴索组均高于对照组（q值分别为33.639、18.788和17.879，P值均〈0.01）；地塞米松2剂组高于1剂组（q=14.8To explore the effects and mechanism of dexamethasone and ambroxol on expression of surfactant protein （SP）-B mRNA and thyroid transcription factor （TTF）-1 in premature rat lung. Methods Sixteen pregnant Sprague-Dawley rats were randomly divided into four equal groups： two doses of dexamethasone （0.2 mg/kg injected intramuscularly on Day 17 and 18 of pregnancy respectively）;single dose of dexamethasone （0.2 mg/kg injected intramuscularly on Day 18 of pregnancy）;ambroxol group （100 mg/kg injected intraperitoneally on Day 16, 17 and 18 of pregnancy respectively）; and control group （normal saline injected intraperitoneally on Day 16, 17 and 18 of pregnancy respectively）. There were four pregnant rats in each group. All of the fetal rats were taken out on Day 19 of pregnancy as the preterm birth model, and 20 fetal rats from each group were randomly selected. The ratio of body weight to fetal lung weight of newborn rats was calculated. Changes in lung morphology were observed under light microscopy and the ratio of alveoli surface area to alveolar septae surface area was calculated. Expression of TTF-1 protein was determined by immunohistochemistry. Expression of SP-B mRNA was detected by reverse transcriptase-polymerase chain reaction. One-way analysis of variance, Student-Newman-Keuls method and Pearson correlation analysis were applied as statistical methods. Results （1） The ratio of body weight to fetal lung weight was （6.5±0.6）, （7.9±0.8）, （9.5±0.8） and （9.5±0.9） mg/g in two doses of dexamethasone group, one dose of dexamethasone group, ambroxol group and control group respectively （F=67.69,P〈0.01）. The ratio of two doses and one dose of dexamethasone group was lower than that of control group （q=17.143 and 9.143, all P〈0.01） and ambroxol group （q=17.143 and 9.143, all P〈0.01）. The ratio of two doses dexamethasone group was lower than that of one dose dexamethasone group （q=8.000, P〈0.01）. （2） The ratio of alveoli surface area to alveo

关 键 词：地塞米松 氨溴索 肺表面活性物质相关蛋白质 B 核蛋白质类 转录因子 婴儿  早产 

分 类 号：R722.6[医药卫生—儿科]