羊膜衍生膜负载骨髓间充质干细胞修复软骨缺损的可行性研究  

The feasibility study of repairment of articular cartilage defects with bone marrow-derived mesenchymal stem cells seeded on acellular amniotic membrane

作　　者：陈浩[1] 张治金[2] 赵德伟[1] 郭林[1] 

机构地区：[1]大连大学附属中山医院骨二科,辽宁省大连市116001 [2]杭州市萧山区中医院骨科

出　　处：《中华显微外科杂志》2014年第3期254-257,共4页Chinese Journal of Microsurgery

摘　　要：目的研究人羊膜衍生膜负载骨髓间充质干细胞修复兔膝关节非负重区软骨缺损的可行性。方法自2012年7月至2013年3月，体外分离、扩增兔BMSCs，以1．63×10^5／cm^2的密度接种于HAAM上，共培养7～8d后，行电镜、光镜检查及苏木精-伊红（HE）染色。24只兔股骨髁间窝制备直径4mm、深3mm的软骨缺损模型，随机分为A、B两组，每组12只，建立膝关节髁间窝非负重区关节软骨缺损模型，右膝为空白对照侧，不植入任何材料，左膝为实验侧。A组左膝植入BMSCs与HAAM复合体，B组左膝植入单纯HAAM。移植4、12周后取新生组织，以行大体观察、组织学检查。结果大体观察及组织学观察，术后4、12周A组BMSCs—HAAM侧再生组织色白、质软、表面光滑，可见透明软骨细胞。12周时修复组织与正常软骨平齐，修复部位表面较平整，界限模糊，接近正常软骨；修复组织全层可见透明软骨细胞。B组HAAM侧修复组织表面不平并有明显凹陷，主要由成纤维细胞组成。两组空白对照侧未见明显修复。结论人羊膜衍生膜负载BMSCs可以修复兔膝关节髁间窝非负重区关节软骨缺损。Objective To study the effect of repairment of articular cartilage defects in non weight-beating area of rabbit with bone marrow-derived mesenehymal stem cells （ BMSCs ） seeded on human acellular amniotic membrane（HAAM）. Methods From July 2012 to March 2013, bone marrow-derived mesenehymal stem cells were isolated and purified from rabbit in vitro. The cells were seeded on human aeellular amniotic membrane at the concentration of 1.63×10^5/cm^2. From 7 days to 8 days after cultured, the complexes of BMSC and HAAM were examined under electronmieroseope, light microscope and by HE stain. Full thickness empty defects measuring 4 mm in diameter by 3 mm depth were prepared in femoral intereondylar fossa of 24 rabbits. The rabbits were randomized into two groups ： group A and group B with 12 each group. The defects of fight knees were served as control and the left as experimental group. BMSCs/HAAM composite was cultured and then transplanted into the defect of left knee joint in group A as group BMSCs/HAAM and HAAM into group B as group HAAM. These rabbits were killed at 4 and 12 weeks after surgery in each group and the newly cartilage samples were evaluated grossly, histologically are graded. Results In the 4th and 12th week after the operation, the regenerated tissue were white, soft and smooth. Chondrocytes were found in the tissue. In the 12th week, the morphology, distribution and arrangement of the regenerated tissues were similar to normal cartilage in the knees with HAAM-BMSCs transplantation. The regenerated tissues grew to be integrated with the surrounding normal cartilage with obscure boundary between them. Chondroeytes were found in all layer of the tissue, surrounding normal cartilage with obscure boundary between them. In the HAAM transplantation, the rough surface of regenerated tissue sunk obviously and the fibmblasts in all layer were found. While there were no regenerated tissue in the control side. Conclusion BMSCs seeded on HAAM could repair the articular cartilage defects of femoral i

关 键 词：人羊膜衍生膜 骨髓间充质干细胞 软骨缺损 

分 类 号：R68[医药卫生—骨科学]