Effect of Honghua (Flos Carthami) on nitric oxide production in RAW 264.7 cells and α-glucosidase activity  

作　　者：Hui Liao Linda Banbury Hongping Liang Xiaomin Wang Xiaokai Lü Ling Hu Jin Wu 

机构地区：[1]Department of Pharmacy, Shanxi Provincial People's Hospital [2]Southern Cross Plant Science, Southern Cross University [3]Department of Clinical Laboratory, Shanxi Provincial People's Hospital [4]Department of Pharmacy,Shanxi DaYi Hospital [5]Department of Endocrinology, Shanxi Provincial People's Hospital [6]Management of Hospital, Shanxi Medical University Second Hospital

出　　处：《Journal of Traditional Chinese Medicine》2014年第3期362-368,共7页中医杂志（英文版）

基　　金：Supported by Shanxi Scholarship Council of China(No.2010-102)

摘　　要：OBJECTIVE： To study the effects of extracts from Honghua （Flos Carthaml~ on lipopolysaccharide in- duced nitric oxide （NO） production in RAW 264.7 cells and the influence of the extracts on yeast a-glucosidase activity. The total flavonoid content of the extracts was also determined. METHODS： Cytotoxicity of the extracts to RAW 264.7 cells was evaluated by the ATPliteTM method. Inhibitory effects of the extracts on NO production were evaluated by Griess assay. Curcumin was used as a positive control. Screening of extracts for po- tential a-glucosidase inhibitors was done by a fiuo- rometric assay. The assay was based on the hydroly- sis of 4-methylumbelliferyl-a-D-glucopyranoside toform the fluorescent product, 4-methylumbellifer- one. Acarbose was used as a positive control. The total t3avonoid content was tested using kaempfer- ol as the standard. RESULTS： There were significant inhibitory effects on NO production when the extracts were 25-100 μg/ mL （P〈0.05） and curcumin was 2-4 μg/mL （P〈 0.001）. The extracts showed an inhibitory effect on a-glucosidase activity at the concentrations of 15.6-125 μg/mL with a half maximal （50%）inhibito- ry concentration （ICs0） of （32.8± 5.7） μg/mL, com- pared with the ICs0 of acarbose at （1.8±0.4） μg/mL. There was a significant difference between the two IC50 values （P〈0.001）. The total content of flavo- noids per gram of dried herb was 1.14 mg. CONCLUSION： Honghua （Flos Carthami） showed in- hibitory effects on NO production in activated RAW 264.7 macrophage cells and an inhibitory effect on yeast a-glucosidase. There might be a relationship between these pharmacological effects and its fla- vonoid content.OBJECTIVE: To study the effects of extracts from Honghua(Flos Carthami) on lipopolysaccharide induced nitric oxide(NO) production in RAW 264.7cells and the influence of the extracts on yeastα-glucosidase activity. The total flavonoid content of the extracts was also determined.METHODS: Cytotoxicity of the extracts to RAW264.7 cells was evaluated by the ATPlite? method.Inhibitory effects of the extracts on NO production were evaluated by Griess assay. Curcumin was used as a positive control. Screening of extracts for potential α-glucosidase inhibitors was done by a fluorometric assay.The assay was based on the hydrolysis of 4-methylumbelliferyl-a-D-glucopyranoside to form the fluorescent product, 4-methylumbelliferone. Acarbose was used as a positive control. The total flavonoid content was tested using kaempferol as the standard.RESULTS: There were significant inhibitory effects on NO production when the extracts were 25-100μg/mL(P<0.05) and curcumin was 2-4 μg/mL(P<0.001). The extracts showed an inhibitory effect onα-glucosidase activity at the concentrations of15.6-125 μg/mL with a half maximal(50%) inhibitory concentration(IC50) of(32.8±5.7) μg/mL, compared with the IC 50 of acarbose at(1.8±0.4) μg/mL.There was a significant difference between the two IC50values(P<0.001). The total content of flavonoids per gram of dried herb was1.14 mg.CONCLUSION:Honghua(Flos Carthami)showed in hibitory effects on NO production in activated RAW264.7 macrophage cells and an inhibitory effect on yeast α-glucosidase. There might be a relationship between these pharmacological effects and its flavonoid content.

关 键 词：Carthamus tinctorius Nitric oxide AI-pha-glucosidases FLAVONOIDS RAW 264.7 cells 

分 类 号：R285[医药卫生—中药学]