GATA-4在小鼠生殖腺中的表达定位研究  

Localization Study of GATA- 4 Expression in the Genital Gland of Mouse

作　　者：景彩霞[1] 杨加周[2] 庞秋霞[3] 艾庆燕[2] 王艳梅[2] 赵豫凤[2] 

机构地区：[1]延安大学医学院寄生虫教研室,陕西延安716000 [2]延安大学医学院组织胚胎教研室,陕西延安716000 [3]延安大学医学院生物化学教研室,陕西延安716000

出　　处：《延安大学学报（医学科学版）》2014年第2期12-14,共3页Journal of Yan'an University:Medical Science Edition

基　　金：陕西省延安市科学技术研究发展计划项目(2011ks-19;2011ks-33)

摘　　要：目的研究小鼠睾丸和卵巢内GATA-4免疫反应产物的特征与分布。方法选取6周雄性和雌性小鼠各6只,分别取其睾丸、卵巢制作石蜡切片,应用免疫组织化学ABC法,DAB显色,观察GATA-4在睾丸和卵巢中的表达情况。结果 GATA-4免疫反应产物在这个阶段小鼠睾丸Sertoli细胞、Leydig细胞、生精细胞和卵巢颗粒细胞、卵泡内膜细胞中均有特征性分布。结论小鼠睾丸和卵巢内均有GATA-4蛋白的表达,说明GATA-4在睾丸性别决定与分化、卵泡发育和激素调控方面具有一定的作用。Objective To explore the characteristics and distribution of GATA - 4 in genital glands of mouse. Meth- ods six - week old female mice and six - week old male mice were selected for this study. Paraffin sections of the o- varies were made and expressions of GATA -4 were observed by the immunohistochemical ABC method with DAB the visualization. Results Positive expressions of GATA -4 were found in Sertoli cells and Leydig cells and germ cells, granulosa cells and thecal cells of the mice. Conclusion This experiment proved that GATA - 4 exists in geni- tal glands of mouse, which provides morphological basis for sex determination and differentiation, follicular develop- ment and hormone regulation in the genital glands.

关 键 词：GATA-4 生殖腺 免疫组织化学 小鼠 

分 类 号：R697[医药卫生—泌尿科学] R715.2[医药卫生—外科学]