五味子特殊类型白果五味子的分子标记研究  

Molecular Characters of Schisandra chinensis with White Fruit by RAPD and ISSR Makers

作　　者：李先宽[1] 王冰[1] 郑艳超[1] 宋新[1] 吴亚男[1] 陈靓[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中药材》2014年第4期568-572,共5页Journal of Chinese Medicinal Materials

基　　金：国家科技基础性工作专项重点项目(2007FY1106002010);辽宁省教育厅项目(LT2010067)

摘　　要：目的:应用RAPD、ISSR两种标记技术在分子水平上表现白果五味子的特点,并对种群遗传多样性和遗传分化进行研究。方法:12个RAPD随机引物和8个ISSR引物对21个个体进行检测,应用POPGENE32软件对结果进行处理。结果:引物S83、S180、S300对白果五味子样品可以扩增出区别于五味子样品的某一或某些特有条带。RAPD标记共得到66个可重复的位点,白果五味子类型的多态性位点52个,多态位点百分率为78.79%;ISSR标记共得到42个可重复的位点,其中多态性位点25个,白果五味子类型的多态位点百分率为59.52%。和正常五味子种群相比,白果五味子类型遗传并不稳定,具有更丰富的遗传多样性,但两种群之间还没有形成较大的遗传差距。结论:RAPD、ISSR两种分子标记技术,可以在分子水平上表现白果五味子的特点,并可揭示五味子种质资源的亲缘关系。Objective： The characters of Schisandra chinensis with white fruit were represented at molecular levels and the genetic diversity were investigated using RAPD and ISSR.Methods： 12 primers of RAPD randomized markers and 8 primers of ISSR markers were used to test 21 samples of white fruit Schisandra chinensis,and POPGENE 32 software were used to analyze the results.Results：One or more unique bands were produced to distinguish white fruit Schisandra chinensis from normal Schisandra chinensis using the primers of S83,S180 and S300.RAPD： 66 discernible DNA fragments were generated with 52（ 78.79%） polymorphic fragments; ISSR：42 discernible DNA fragments were generated with 25（ 59.52%） polymorphic fragments.The genetic variation of white fruit Schisandra chinensis was more unstable than normal Schisandra chinensis,but the genetic distance of them was small at the species level.Conclusion： RAPD and ISSR markers can be used to put up the characteristics of Schisandra chinensis with white fruit at molecular levels.Also they can indicate the genetic relationship of the Schisandra chinensis germplasm resource.

关 键 词：白果五味子 遗传多样性 RAPD ISSR 种群 

分 类 号：R282.1[医药卫生—中药学]