补骨脂酚对ESF-1细胞抗衰老基因调控机制研究  

Regulative Effect of Bakuchiol on ESF-1 Cells Anti-aging Gene

作　　者：于茜[1] 邹海曼[1] 王帅[1] 徐艳明[1] 李建民[1] 张宁[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中药材》2014年第4期632-635,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81274035);黑龙江省自然科学基金面上项目(D201234);高等学校博士学科点专项科研基金(20112327120009);教育部"春晖计划"合作科研项目(Z2010018)

摘　　要：目的:通过研究补骨脂酚对人真皮正常成纤维细胞(ESF-1)增殖活性以及抗老化相关蛋白mRNA表达水平的影响,探讨补骨脂酚延缓皮肤衰老的机制。方法:将体外培养的正常ESF-1细胞分为空白对照组、雌二醇(10-3μmol/L)阳性对照组及补骨脂酚高、中、低剂量(100、10、1μmol/L)组,采用MTT法检测细胞活性,逆转录聚合酶链式反应(RT-PCR)法检测药物作用后细胞中Ⅰ型胶原蛋白(ColⅠ)、Ⅲ型胶原蛋白(ColⅢ)、基质金属蛋白酶抑制剂1(TIMP-1)、基质金属蛋白酶抑制剂2(TIMP-2)、基质金属蛋白酶1(MMP-1)mRNA的表达水平。结果:雌二醇和中剂量补骨脂酚能促进ESF-1细胞的增殖,显著上调ColⅠ、ColⅢ、TIMP-1、TIMP-2 mRNA的表达,下调MMP-1 mRNA的表达。结论:补骨脂酚能促进ESF-1细胞的增殖,促进胶原蛋白和基质金属蛋白酶抑制剂mRNA的表达,并能抑制基质金属蛋白酶mRNA的表达,从而发挥抗皮肤衰老的作用。Objective： To explore the mechanism of bakuchiol on anti-aging gene mRNA expression level of human skin fibroblasts（ ESF-1）.Methods： The potential of cell proliferation which was divided into blank group,positive control estradiol group,and bakuchiol high,medium and low dose groups was detected by MTT.The expression levels of ColⅠ,Col Ⅲ,TIMP-1,TIMP-2 and MMP-1 mRNA were detected with RT-PCR.Results： ESF-1 vitality and the expression levels of ColⅠ,ColⅢ,TIMP-1 and TIMP-2 mRNA were significantly increased by bakuchiol and E2.However,the expression of MMP-1 mRNA was reduced by bakuchiol and E2.Conclusion： The bakuchiol can enhance ESF-1 cell activity,promote collagen and matrix metalloproteinase inhibitors mRNA expression level and inhibit mRNA expression of matrix metalloproteinases in order to postpone skin aging.

关 键 词：补骨脂酚 ESF-1细胞 抗皮肤衰老 

分 类 号：R285.5[医药卫生—中药学]