甘青乌头总黄酮提取精制工艺研究  

Extraction and Purification Technologies of Total Flavonoids from Aconitum tanguticum

作　　者：李艳茸[1,2] 杨立新[2,3] 冯伟红[2,3] 李春[2,3] 王智民[1,2,3] 

机构地区：[1]陕西中医学院,陕西西安712046 [2]中国中医科学院中药研究所,北京100700 [3]中药质量控制技术国家工程实验室,北京100700

出　　处：《中药材》2014年第4期670-674,共5页Journal of Chinese Medicinal Materials

基　　金：北京市自然科学基金(7132152);中央级公益性科研院所基本科研业务费专项资金资助(ZZ070828)

摘　　要：目的:优选甘青乌头总黄酮的提取精制工艺。方法:以总黄酮含量为检测指标,采用正交设计考察乙醇浓度、乙醇加入量、提取时间和提取次数对总黄酮提取率的影响,优选甘青乌头总黄酮的提取工艺;以总黄酮吸附量(mg/g)和解析率(%)为检测指标,考察D101、AB-8、X-5和XAD-16四种大孔吸附树脂对甘青乌头总黄酮的富集能力,优选树脂类型,并确定上样液浓度、上样流速、上样量、洗脱溶媒和洗脱流速等工艺参数。结果:甘青乌头总黄酮最佳提取精制工艺为:10倍量70%乙醇回流提取3次,每次提取1 h;优选XAD-16树脂精制甘青乌头总黄酮,最佳上样液浓度为8 mg/mL,上样量为112 mg/g干树脂,上样液pH值为5,上样流速为3 mL/min,最佳洗脱溶媒为70%乙醇,洗脱流速为5 mL/min;在最佳吸附解析条件下,甘青乌头总黄酮质量分数从4.39%提高到46.19%。结论:甘青乌头总黄酮提取精制工艺操作简单,结果可靠,可用于工业化生产。Objective： To optimize the extraction and purification technologies of total flavonoids from Aconitum tanguticum whole plant.Methods： With the content of total flavonoids as index,the optimum extraction conditions for the concentration,volume of alcohol,extracting time and times were selected by orthogonal optimized; Comparing the adsorption quantity（ mg /g） and resolution（ %）,four kinds of macroporous adsorption resins including D101,AB-8,X-5 and XAD-16 were investigated for the enrichment ability of total flavonoids from Aconitum tanguticum; Concentration and pH value of sample,sampling amount,elution solvent and loading and elution velocity for the optimum adsorption resin were determined.Results： The content of total flavonoids in Aconitum tanguticum was about4.39%; The optimum extraction technique was 70% alcohol reflux extraction for three times,each time for one hour,the ratio of material and liquid was 1∶ 10（ w /v）; The optimum purification technology was： using XAD-16 macroporous resin,the initial concentration of total flavonoids of Aconitum tanguticum was 8 mg /mL,the sampling amount was 112 mg /g dry resin,the pH value was 5,the loading velocity was 3 mL /min,the elution solvent was 70% ethanol and the elution velocity was 5 mL /min.Under the optimum conditions,the average content of total flavonoids was raised from 4.39% to 46.19%.Conclusion： The optimum extraction and purification technologies for total flavonoids of Aconitum tanguticum were suitable for industrial production for its simplicity and responsibility.

关 键 词：甘青乌头 总黄酮 含量测定 提取精制工艺 

分 类 号：R286[医药卫生—中药学]