大麻素CB1受体和纹状体多巴胺D2受体参与电针镇痛机制  

INVOLVEMENT OF CANNABINOID RECEPTOR-1 AND DOPAMINE D2 RECEPTOR IN CORPUS STRIATUM IN SINGLE AND REPEATED ELECTROACUPUNCTURE-INDUCED ANALGESIA

作　　者：寿崟[1] 赵颖倩 徐鸣曙[3] 葛林宝 张必萌[1] 

机构地区：[1]上海交通大学附属第一人民医院针灸科,上海200080 [2]陕西中医学院针灸推拿学院,咸阳712046 [3]上海市针灸经络研究所脑神经生物实验室,上海200030 [4]上海市气功研究所,上海200030 [5]上海市针灸经络研究中心,上海201203

出　　处：《中国疼痛医学杂志》2014年第6期388-392,共5页Chinese Journal of Pain Medicine

基　　金：国家重点基础研究发展计划(973计划)(2009CB522901);上海市科学技术委员会科研计划项目课题基金资助项目(07DZ19722-5)

摘　　要：目的:研究大麻素CB1受体和多巴胺D2受体是否参与单次或反复电针镇痛机制。方法:以完全弗氏佐剂(complete Freund's adjuvant,CFA)造成佐剂性关节炎大鼠模型,分别对单次及反复电针后的大鼠进行痛阈和纹状体D2受体mRNA表达水平检测。同时用CB1受体拮抗剂或激动剂干预。结果:①单次和反复电针后痛阈均升高,且关节炎痛+电针+拮抗剂组的镇痛效果弱于关节炎痛+电针组(P<0.01);关节炎痛+激动剂组与关节炎痛+电针组镇痛效果比较,差异无统计学意义。②无论是单次或反复电针,关节炎痛+电针+拮抗剂组大鼠纹状体D2受体mRNA表达水平显著低于电针组(P<0.01)。③在反复电针观察组中,关节炎痛+激动剂组大鼠纹状体D2受体mRNA表达水平与关节炎痛组相比,差异无统计学意义,显著低于关节炎痛+电针组(P<0.01)。结论:在本实验条件下,单次和反复电针产生的镇痛作用可能部分通过大麻素受体CB1介导,纹状体D2受体可能也参与其中。Objective：To observe whether the cannabinoid receptor-1 （CB1） and dopamine D2 receptor are involved in analgesic effect evoked by single or repeated electroacupuncture （EA）.Methods：In the complete Freund＇s adjuvant-induced arthritis rats,paw withdrawal latency was measured after single or repeated EA.The D2 receptor mRNA in the corpus striatum was assessed by quantitative real time polymerase chain reaction （qRT-PCR）.Intraperitoneal injection ofcannabinoid CB1 receptor selective antagonistAM251 and agonist WIN55212-2 was applied to the relevant groups.Results：① The paw withdrawal latency was prolonged after single or repeated EA,the analgesic effects in M＋EA＋AM251 group were inferior to the M＋EA group （P 〈 0.01）.There was no significant difference in analgesic effects between M＋WIN55212-2 group and M＋EA group.② In either case,M＋EA＋AM251 group showed significantly lower D2 receptor mRNA level compared with that of M＋EA group （P 〈 0.01）.③ In the repeated EA experiment,there was no significant difference in D2 receptor mRNA expression between M＋WIN55212-2 group and M group.However,the expression level of D2 receptor was significantly lower than that in M＋EA group （P 〈 0.01）.Conclusion：Under the experimental condition,single or repeated EA induced analgesia might be mediated partly by CB 1 receptor.The D2 receptor in the corpus striatum may also be involved.

关 键 词：电针 镇痛 大麻素CB1受体 多巴胺D2受体 

分 类 号：R245[医药卫生—针灸推拿学]