支气管肺泡灌洗液与咽拭子荧光定量PCR法检测成人肺炎支原体感染的对比研究  

A Comparative Study of Detection Between BALF and Swabs Samples by Real-time PCR in Adults Patients with MPP

作　　者：金欣[1] 于农[1] 陈水平[1] 左向华[1] 佟利威 许晓跃[1] 吴海涵[1] 姜雪丹[1] 袁方[1] 李占甲[1] 

机构地区：[1]军事医学科学院附属医院检验科,北京100071

出　　处：《标记免疫分析与临床》2014年第3期301-302,327,共3页Labeled Immunoassays and Clinical Medicine

基　　金：国家高技术研究发展计划子课题(863计划)(2011AA02A109)

摘　　要：目的 用实时荧光定量PCR方法对肺部感染危重患者的咽拭子样本和支气管肺泡灌洗液（BALF）进行检测,探讨不同样本对检测结果的影响.方法 450例肺部感染危重患者纳入临床研究,用实时荧光定量PCR方法检测患者咽拭子样本和BALF样本,对检测结果进行比较.结果 在450例肺部感染危重患者中,肺炎支原体肺炎（MPP）患者1 15例,占25.6％.BALF样本检测的阳性率为69.56％,咽拭子样本检测的阳性率为13.04％.肺炎支原体（MP）感染的年龄段主要在20～39和50 ～59岁,以50-59岁年龄段人数最多.结论 MP是肺部感染主要病原体之一,采集BALF标本用实时荧光PCR检测MPP阳性率更高,是MPP诊断的有效手段.Objective To investigate the effect of different samples between swabs and bronchotracho alveolar larvage by real-time PCR in patients with community acquired pneumonia.Methods Clinical data,including 450 patients with severe pulmonary infection,samples of swabs and bronchotracho alveolar larvage were detected by real-time PCR.Results 115 patients were diagnosed with MPP （25.6%） in 450 patients of severe pulmonary infection.The positive rates were 69.56% and 13.04% in samples of BALF and swab,respectively.MP infection were frequent between patients aged 20-29 and 50-59 years.The big numbers of patient were most in age 50-59 years.Conclusion Mp remains one of the major pathogens in pulmonary infection.The positive rate of BALF detection is higher than that of swabs detection.The method of real-time PCR is effective means of diagnosis MPP.

关 键 词：肺炎支原体肺炎 实时荧光定量PCR 支气管肺泡灌洗液 

分 类 号：R725.6[医药卫生—儿科]