欧洲黑杨PnEXPA1基因多态性与稳定碳同位素比率的关联分析  

Association Analysis of Polymorphisms in PnEXPA1 Gene with Carbon Isotope Ratios in Black Poplar( Populus nigra L. )

作　　者：张伟溪[1] 褚延广[1] 黄秦军[1] 张冰玉[1] 丁昌俊[1] 苏晓华[1] 

机构地区：[1]林木遗传育种国家重点实验室中国林业科学研究院林业研究所、国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学研究》2014年第3期349-355,共7页Forest Research

基　　金：国家自然科学基金项目(31000310);国家“十一五”科技支撑项目(2006BAD01A15)

摘　　要：对欧洲黑杨α-expansin基因PnEXPA1的SNP多态性与水分利用效率相关性状稳定碳同位素比率(δ13C)进行了关联分析。利用SNaPshot技术对PnEXPA1基因内11个SNP位点进行了基因型分型,发现各SNP位点优势基因型均为纯合,且其频率高于杂合基因型。关联分析显示:SNP8和SNP12在采用单因素方差分析(ANOVA)和一般线性模型(GLM)2种方法时均与δ13C值显著关联。SNP8为exon 1内的无义突变,可解释6.620%的表型变异;而SNP12位于intron 1中,遗传贡献率为6.613%;这2个SNP位点与SNP9、SNP13共同位于一个高连锁不平衡(LD)的单倍型块中。SNP8与SNP12的TT基因型无性系均具有较高的δ13C值,为优势基因型。The authors report an association study of SNP polymorphisms in a α-expansin gene PnEXPA1 with stable carbon isotope ratios （δ^13C） in Populus nigra. In total, 11 SNP loci were genotyped with SNaPshot technology which indicates that homozygous genotype is more commonly ocurred for each individul with higher allele frequency. Association analysis under ANOVA and GLM methods revealed that SNP8 and SNP12 were associated with δ^13C values. SNP8 was a synonymous mutation located in exon 1 and could explain 6. 620% of genetic variation of trait, while SNP12 positioned in intronl and contributed to 6. 613% of genetic variation of trait. These two SNPs, together with SNP 9 and SNP13, were located in a haplotype block with higher LD levels. Clones with TT homozygous at SNP8 and SNP12 were found to have higher values of δ^13C. These results provide insights into the molecular basis of the effects of PnEXPA1 on genetic variation of water use efficiency （WUE）, as well as genic marker assisted breed- ing in poplar.

关 键 词：欧洲黑杨 伸展蛋白基因 单核苷酸多态性 稳定碳同位素比率 关联分析 

分 类 号：S792.119[农业科学—林木遗传育种]