鲭鱼罐头中7种多氯联苯的测定——亚临界水萃取-气相色谱法  

Determination of 7 Polychlorinated Biophenyls in Canned Mackerel——Sub-critical Water Extraction-gas Chromatography Method

作　　者：王婷婷[1] 李莉[1] 陈易晖 徐颖[3] 聂诚刚 刘望才[1] 房江华[1] 高浩其[1] 

机构地区：[1]宁波工程学院,浙江宁波315016 [2]象山出入境检验检疫局,浙江象山310014 [3]北仑出入境检验检疫局,浙江北仑315800

出　　处：《宁波工程学院学报》2014年第2期1-6,共6页Journal of Ningbo University of Technology

基　　金：浙江省自然科学基金(项目编号:LQ12B05001);宁波市自然科学基金(项目编号:2012A610091);宁波市创新团队(2011B82002);北仑科技创新基金和2012校立项(项目编号:46)

摘　　要：建立了同时检测鲭鱼罐头中7种多氯联苯（PCBs）的亚临界水萃取-气相色谱分析方法。亚临界水中加入活性炭纤维为固相萃取剂，在200℃的条件下，提取40 min，随后，用2 mL乙酸乙酯洗脱。结果表明，该方法测定PCBs含量的线性范围为0.001～0.100μg/mL；在2.0、10.0、50.0μg/kg （n=6）的添加水平下，回收率在79.4%～91.0%之间，测定结果的相对标准偏差（RSD）为3.1%～12.3%；检出限（S/N=3）为0.016～0.046μg/kg；定量限（S/N=10）为0.048～0.139μg/kg。该方法绿色环保、稳定、可靠，适用于鲭鱼罐头中多氯联苯的检测。A method for the simultaneous determination of 7 polychlorinated biophenyls （PCBs） in canned Mackerel by sub-critical water extraction coupled with gas chromatography is developed. PCBs are extracted by 200 ℃ sub-critical water for 40 min, and adsorbed on active carbon fibers at the same time. And then, PCBs are desorbed by 2 mL ethyl acetate. The results show that the linear range for the determination of PCBs in canned Mackerel is between 0.001～0.100 μg/mL with the limit of detection （LOD, S/N=3） of 0.016～0.046 μg/kg and the limit of quantification （LOQ, S/N=10） of 0.048～0.139 μg/kg. At the spiked level of 2.0、10.0、50.0 μg/kg （n=6）, the recoveries range from 79.4% to 91.0% with the relative standard deviations （RSDs） between 3.1% and 12.3%. The developed method is environmentally-friendly and suitable for the simultaneous determination of 7 PCBs in canned Mackerel with high accuracy and high precision.

关 键 词：亚临界水萃取 气相色谱 多氯联苯 鲭鱼罐头 

分 类 号：O658[理学—分析化学]