十六烷基三甲基溴化铵强化产氢发酵  

Enhancement of Fermentative Hydrogen Production by Addition of Cetyl Trimethyl Ammonium Bromide

作　　者：任云利[1] 李欣[1] 张柯[1] 杨松波[1] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471023

出　　处：《河南科技大学学报（自然科学版）》2014年第5期95-99,113,共5页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家"973"科技计划基金项目(2011CB211702);河南科技大学SRTP基金项目

摘　　要：微生物发酵产生的生物气要经过细胞膜释放出去，产氢菌胞外膜的通透性影响产氢发酵过程。本文研究了十六烷基三甲基溴化铵（CTAB）对混合菌胞外膜通透性和厌氧产氢发酵过程的影响。研究结果表明：浓度大于0.0095 g/L的CTAB可有效提高混合菌胞外膜的通透性；浓度小于0.0450 g/L时，CTAB对发酵终端的生物量和 pH无明显影响；在0.0095～0.0270 g/L的 CTAB条件下，发酵体系的产氢速率和累计产氢量随着 CTAB浓度的增加而增加；在0.0270 g/L的 CTAB条件下，获得最大产氢速率19.6 mL/h 和最大产氢效率13.6 mmol每克木糖，分别较未添加 CTAB时提高44％和38％；在0.0450 g/L 的 CTAB 条件下，混合菌产氢能力受到明显抑制。这些研究结果表明：合适浓度的 CTAB可以作为一种高效的生物活化剂用于强化厌氧产氢发酵。Biogas evolved by bacteria needs to be released through cell membrane. The fermentative hydrogen production was influenced by the permeability of the outer membrane of hydrogen producing bacteria. In this paper,the permeability of the outer membrane of the mixed microflora and the fermentative hydrogen production with addition of cetyl trimethyl ammonium bromide( CTAB) were investigated. The experimental results show that CTAB with the concentration greater than 0. 009 5 g /L can penetrate the outer membrane of the mixed microflora. CTAB with the concentration less than 0. 045 0 g /L has no notable impact on biomass and pH during final fermentation. In the presence of CTAB with concentration of 0. 009 5 g /L to 0. 027 0 g /L,the H2 production rate and H2 yield are found to increase with increasing CTAB concentrations. At CTAB concentration of 0. 027 0 g /L,the maximum H2 production efficiency of 13. 6 mmol /g xylose and the maximum H2 production rate of 19. 6 mL /h are gained,which are 38% and 44% higher than those of the corresponding blank one,respectively. At CTAB concentration of 0. 045 0 g /L,fermentative hydrogen production is significantly inhibited. These results suggeste that CTAB can be used as a low-cost and highly efficient bioactive agent to improve dark fermentative H2 production.

关 键 词：发酵产氢 十六烷基三甲基溴化铵 胞外膜 通透性 

分 类 号：X382.1[环境科学与工程—环境工程]