人脂肪间充质干细胞移植结合跑台训练对脊髓损伤大鼠运动功能的影响及机制  

Effects of combination of human adipose-derived mesenchymal stem cells transplantation and treadmill training on locomotor functional recovery after incomplete spinal cord injury in rats

作　　者：张鑫[1] 陈银海[2] 

机构地区：[1]南方医科大学珠江医院康复医学科,广州510282 [2]南方医科大学珠江医院门诊部

出　　处：《中国康复医学杂志》2014年第6期504-510,共7页Chinese Journal of Rehabilitation Medicine

基　　金：广东省科技计划项目(2010B080701020)

摘　　要：目的:探讨人脂肪间充质干细胞(hADSCs)结合跑台训练对脊髓损伤(SCI)大鼠运动功能的影响。方法:成年雄性SD大鼠60只,采用改良Allen打击法制作T10不完全SCI模型。术后随机分为损伤组(模型组,未行处理)、细胞移植组(细胞组,行细胞移植)、跑台训练组(训练组,行跑台训练)和跑台训练结合细胞移植组(联合组,行跑台训练及细胞移植)。术前及术后采用BBB评分和Tarlov评分行运动功能评定,术后1、2、4周取脊髓行免疫荧光染色检测细胞存活,炎症反应相关细胞(ED1阳性巨噬细胞),胶质瘢痕[胶质纤维酸性蛋白,GFAP及神经丝蛋白(NF-200),5-羟色胺(5-HT)]。结果:治疗组行为学评分及荧光定量检测均优于模型组(P<0.05);但联合组最明显,训练组和细胞组间无明显区别(P>0.05)。结论:hADSCs移植联合跑台训练对不完全性SCI大鼠运动功能恢复具有协同功效,治疗机制可能与进一步减轻伤后炎症反应,减少损伤面积,促进轴突再生有关。Objective：To investigate the effects of combination of human adipose-derived mesenchymal stem cells（hADSCs） trumsplantation and treadmill training on locomotor function recovery after incomplete spinal cord injury （SCI） in rats.Method：Sixty male adult SD rats were included to make incomplete SCI at T10 level by using modified Allen＇s method.They were divided randomly into control group,cell group （cell transplantation）,exercise group （treadmill training）,and combination group（cell plus exercise）.Before and after SCI,locomotor function was evaluated by using Basso,Beattie and Bresnahan （BBB） scale and Tarlov scale,the cell survival and expressions of ED-1＋,GFAP,NF-200 and 5-HT were detected by immunofluorescence at 1,2,4 weeks post SCI.Result：In treatment groups both BBB scores,Tarlov scores and immunofluorescence detection were better than those of control group,but combination group had the best effects （P ＜ 0.05）,there was no significant difference between the cell and exercise groups （P＞ 0.05）.Conclusion：The combination of hADSCs and treadmill training can yield synergic locomotor functional inprovement after incomplete SCI.It maybe related to alleviating inflammatory reaction,reducing damage area,and promoting axon regeneration.

关 键 词：脊髓损伤 人脂肪间充质干细胞 跑台训练 运动功能 脊髓可塑性 

分 类 号：R651.2[医药卫生—外科学]