苏木含药血清对人肺癌PG细胞增殖周期影响的对比研究  

Effect ofLignum Sappan Containing Serum on the Proliferation Cycle of Human Lung Cancer Cell Line PG: a Comparative Study

作　　者：郭秀伟[1,2] 张培彤[1] 杨栋[1] 乔路敏 马雪曼 

机构地区：[1]中国中医科学院广安门医院肿瘤科,北京100053 [2]北京中医药大学临床医学院,北京100029

出　　处：《中国中西医结合杂志》2014年第6期745-750,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：北京市自然科学基金资助项目(No.7122152)

摘　　要：目的探讨苏木含药血清对人肺癌PG细胞株的周期阻滞作用及其分子机制。方法将肺癌PG细胞分为空白组、苏木组、苏木加顺铂组、顺铂组4组,分别用20%空白血清的1640培养液、20%苏木含药血清的1640培养液、20%苏木含药血清的1640培养液加1μg/mL顺铂、20%空白血清的1640培养液加1μg/mL顺铂培养PG细胞,采用光镜和激光共聚焦显微镜观察各组PG细胞学形态,流式细胞仪检测细胞周期,PCR法检测Rb1、P16 mRNA的表达。结果光镜和激光共聚焦显微镜下苏木组PG细胞的凋亡程度明显,但低于顺铂组和苏木加顺铂组。与空白组比较,苏木组PG细胞的G0/G1和S期比例增加(P<0.05),G2/M期降低(P<0.01);顺铂组和苏木加顺铂组的S和G2/M期比例增加(P<0.05,P<0.01),G0/G1期降低(P<0.01)。与苏木组比较,顺铂组和苏木加顺铂组的S期和G2/M期比例增加(P<0.05,P<0.01),G0/G1期降低(P<0.01)。苏木加顺铂组PG细胞的各期与顺铂组比较,差异无统计学意义(P>0.05)。与空白组比较,苏木组P16、Rb1 mRNA表达增加,顺铂组和苏木加顺铂组亦增加且优于苏木组(P<0.05);与顺铂组比较,苏木加顺铂组P16、Rb1 mRNA表达差异无统计学意义(P>0.05)。结论苏木含药血清通过上调P16、Rb1 mRNA转录水平引起PG细胞G0/G1、S期阻滞,从而诱导细胞凋亡,这与顺铂通过cyclinB1使细胞停滞在G2/M、S期不同。Objective To explore the effect ofLignum Sappan （LS） containing serum on the proliferation cycle arrest of human lung cancer cell line PG and its molecular mechanism. Methods The lung cancer PG cells were divided into four groups, i.e., the blank control group, the LS group, the LS plus cisplatin group, and the cisplatin group. They were cultured by RPMI-1640 with 20% blank serum ,RP- M1-1640 with 20% LS containing serum ,RPMI-1640 with 20% LS containing serum plus 1μg/mL cisplatin, and RPMI-1640 with 20% blank serum plus 1 ~g/mL cisplatin, respectively. The morphology of PG cells was observed using light microscope and laser scanning confocal microscope in each group. The cell cycle arrest was observed using flow cytometry. The expression of P16 and Rbl mRNA was tested by PCR method. Results Under the light microscope and laser scanning confocal microscope, the apoptosis degree of PG cells in the LS group was significant, but less than that of the LS plus cisplatin group as well as the cisplatin group. Compared with the blank control group, the proportion of PG cells increased at G0/G1 and S phases （P 〈0.05） and decreased at G2/M phase （P 〈0.01） in the LS group; The proportion of PG cells increased at G2/M and S phases （P 〈0.05, P 〈0.01） and decreased at G0/G1 phase （P 〈0.01 ） in the LS plus cisplatin group as well as the cisplatin group. Compared with the LS group, the proportion of PG cells increased at G2/M and S phases （P 〈0.05, P 〈0.01） and decreased at G0/G1 phase （P 〈0.01 ） in the LS plus cisplatin group as well as the cisplatin group. There was no statistical difference in PG cells at each phase between the cisplatin group and the LS plus cisplatin group （P 〉 0.05）. The expression of P16 and Rbl mRNA increased in the LS group, when compared with the blank control group. They also in- creased in the cisplatin group and the LS plus cisplatin group, higher than that of the LS group （P 〈0.05）. There was no statistical difference in the expression of P1

关 键 词：苏木 含药血清 细胞周期 P16 RB1 

分 类 号：R285.5[医药卫生—中药学]