TRPV2激活对缺氧再灌注时体外培养星形胶质细胞神经生长因子释放的影响  

TRPV2 Activation Enhances the Expression of Nerve Growth Factor in Primary Cultured Astrocytes Under Oxygen-glucose Deprivation/Reoxygenation

作　　者：肖君[1] 杨斐斐[1] 张函[1] 王伟[1] 何丹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《中国细胞生物学学报》2014年第6期773-779,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81301126;81030021);中国博士后科学基金(批准号:2013M531699)资助的课题~~

摘　　要：神经生长因子对脑缺血后神经元的存活有重要意义。该研究观察了TRPV2激活剂2APB对体外缺血再灌注模型中原代培养大鼠大脑皮层星形胶质细胞神经生长因子释放的影响。将原代培养大鼠大脑皮层星形胶质细胞分为2APB组(0.5 mmol/L)和对照组(不含2APB),在糖氧剥夺情况下培养2 h,然后恢复正常全培养基复氧培养48 h。用Western blot检测星形胶质细胞神经生长因子的表达水平;用ELISA检测星形胶质细胞条件培养液中神经生长因子的含量。结果表明,0.5 mmol/L 2APB可以诱导正常情况下及糖氧剥夺再灌注情况下体外培养星形胶质细胞NGF的合成和释放(P<0.01)。此外,JNK阻滞剂可抑制糖氧剥夺再灌注情况下2APB诱导的星形胶质细胞神经生长因子的释放。综上,TRPV2激活可以影响糖氧剥夺再灌注情况下体外培养星形胶质细胞神经生长因子的合成和释放。TRPV2有可能成为脑缺血再灌注后的潜在治疗靶点。Nerve growth factor （NGF） is essential for neuronal growth and survival. We had addressed the effect of 2APB, an activator of TRPV2, on the NGF expression in astrocytes exposed to oxygen-glucose deprivation/ reoxygenation. Primary cultured cortical astrocytes stimulated with or without 2APB （0.5 mmol/L） were treated with glucose-deprivation media under oxygen-glucose deprivation for 2 h and then incubated under normal culture condition for 48 h. The levels of NGF protein in astrocytes were analyzed by Western blot and NGF protein levels in astrocyte-conditioned media were measured by enzyme-linked immunosorbent assay （ELISA）. 2APB increased the expressions of NGF protein in astrocytes under normal and reoxygenation condition. After 48 h reoxygenation,0.5 mmol/L of 2APB enhanced the levels of NGF protein in astrocyte-conditioned media by 1.88-fold of the control. An inhibitor of c-Jun N-terminal kinase （JNK） suppressed the effect of 2APB on the NGF expression. We concluded that 2APB enhanced, via the JNK pathway, NGF expression in astrocytes. TRPV2 activation might have therapeutic potential for brain injury in ischemia/reperfusion.

关 键 词：星形胶质细胞 糖氧剥夺 再灌注 TRPV2 神经生长因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]