维生素A对体外培养人脐带间充质干细胞生长的影响  

Effects of Retinol(Vitamin A) on Biological Characterization of Mesenchymal Stem Cells from Human Umbilical Cord In Vitro

作　　者：刘丹[1] 余树民[1] 刘欢欢[1] 宋品[1] 曹随忠[1] 沈留红[1] 张坤[1] 左之才[1] 邓俊良[1] 任志华[1] 王娅[1] 马晓平[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014

出　　处：《中国细胞生物学学报》2014年第6期810-817,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31172379);四川省学术与技术带头人培养基金资助的课题~~

摘　　要：该实验以人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs)为研究对象,探讨维生素A对其体外培养的影响。结果显示,添加维生素A培养后,hUCMSCs仍维持其本身生物学特性,表达其标记基因CD29、CD44和干细胞标记基因Oct4、Sox2、Nanog。维生素A促进hUCMSCs的体外增殖,上调增殖基因PCNA、C-myc和干细胞标记基因Nanog的表达,下调凋亡基因Bcl-x的表达。该研究证明了维生素A具有促进hUCMSCs增殖和维持其干细胞特性的作用,对继续探索hUCMSCs的体外快速增殖和维生素A对hUCMSCs增殖调控的机理具有重要意义。This work was designed to investigate the effects of vitamin A on the proliferation of human umbilical cord mesenchymal stem cells （hUCMSCs）. The results showed that after in vitro treatment ofhUCMSCs with vitamin A, the biological characteristics of hUCMSCs could be performed. The expression patterns of hUCMSCs markers and stem cell markers, including CD29, CD44, Oct4, Sox2 and Nanog, were also detected. In addition, we confirmed that vitamin A could stimulate the proliferation of hUCMSCs. The transcriptions of biomolecules, such as PCNA, C-myc and Nanog, were significantly elevated, while the transcript of Bcl-x declined. It was identified that the vitamin A could trigger the characteristics maintenance and proliferation of hUCMSCs, which might be crucial to reveal the regulation mechanism of vitamin A on the proliferation ofhUCMSCs.

关 键 词：人脐带间充质干细胞(hUCMSCs) 维生素A 增殖 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]