京尼平苷干预体外培养大鼠软骨细胞Ⅱ型胶原蛋白的合成  

Influence of geniposide on collagen II synthesis of cultured chondrocytes in vitro

作　　者：谭玉林[1] 鲍同柱[2] 柳琴[2] 韩钰[3] 王艳林[3] 刘扬[2] 周纳新[2] 焉飞 赵龙桃[2] 

机构地区：[1]三峡大学第一临床医学院,湖北省宜昌市443002 [2]宜昌市中心人民医院,湖北省宜昌市443002 [3]三峡大学分子学生物研究所,湖北省宜昌市443002

出　　处：《中国组织工程研究》2014年第20期3149-3152,共4页Chinese Journal of Tissue Engineering Research

基　　金：湖北省自然科学基金(2010CDB10701)~~

摘　　要：背景:近年来的研究表明,中药栀子可能具有活化软骨细胞的功能,而京尼平苷是栀子中重要成分之一。目的:观察京尼平苷对体外培养大鼠膝关节软骨细胞Ⅱ型胶原蛋白合成的影响。方法:体外分离培养大鼠膝关节软骨细胞,分别应用25,50及100 mmol/L京尼平苷干预,并设置正常对照组。RT-PCR和Western blot法观察京尼平苷对各组细胞Ⅱ型胶原mRNA和蛋白表达的影响。结果与结论:RT-PCR和Western blot法检测结果显示,与正常对照组相比,25,50及100 mmol/L京尼平苷干预可以明显促进Ⅱ型胶原mRNA及蛋白的表达(P<0.01)。结果证实,京尼平苷可以促进大鼠膝关节软骨细胞Ⅱ型胶原蛋白的合成。BACKGROUND：Chinese herb Cape Jasmine Fruit can activate the chondrocytes and geniposide is an important component in this herb. OBJECTIVE：To investigate the effect of geniposide on col agen II synthesis in rat chondrocytes cultured in vitro. METHODS：Rat chondrocytes were separated and cultured in vitro. The chondrocytes were then interfered with 25, 50 and 100 mmol/L geniposide. Normal control group was also set. Col agen II mRNA and protein expression was detected with semi-quantitative RT-PCR and western blot analysis, respectively. RESULTS AND CONCLUSION：RT-PCR and western blot analysis results showed that, geniposide at 25, 50 and 100 mmol/L increased the col agen II mRNA and protein expression （P〈0.01）. Geniposide can promote the synthesis of col agen II in rat chondrocytes cultured in vitro.

关 键 词：组织构建 软骨组织工程 京尼平苷 Ⅱ型胶原蛋白 软骨细胞 大鼠 组织工程 湖北省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]