三种钴乙醇酸配合物与牛血清白蛋白相互作用的荧光分析  

Interaction Between Three Cobalt(Ⅱ) Glycollates and Bovine Serum Albumin Studied by Fluorescence Spectrum

作　　者：林海彬[1] 周朝晖[2] 王庆华[1] 汪庆祥[1] 

机构地区：[1]闽南师范大学化学与环境科学系 福建省现代分离分析科学与技术重点实验室,漳州363000 [2]固体表面物理化学国家重点实验室,厦门361005

出　　处：《无机化学学报》2014年第7期1559-1566,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.21275127);福建省教育厅基金(No.JA12216)

摘　　要：利用溶液法合成了3种钴乙醇酸配合物,并对配合物cis-[Co2(Hgly)4(2,2′-bpy)2](Hgly=glycolic acid,2,2′-bpy=2,2′-bipyridine)(3)进行了X-射线单晶衍射表征,结果表明:该配合物为三斜晶系,空间群为P21/c,a=1.003 7(3)nm,b=2.022 6(5)nm,c=1.660 1(4)nm,β=119.942(13)°,V=2.920 3(13)nm3。利用荧光光谱法分别研究了室温下这3种配合物与BSA相互作用的结果,并测定了不同温度下配合物2和3与牛血清白蛋白相互作用的荧光强度的变化,确定了配合物2和3对牛血清白蛋白的荧光淬灭方式均为静态淬灭;同时分析了这两种配合物与牛血清白蛋白相互作用时的结合常数、结合位点数以及热力学函数与温度变化之间的关系,进一步讨论了这两种配合物分别与BSA相互作用时的作用力类型。综合以上结果表明:当分别含有邻菲咯啉和2,2′-联吡啶配体的配合物与BSA相混合时,它们都能与BSA相互作用,且主要的作用力都是氢键和范德华力,但它们对蛋白质中色氨酸的构象或其周围环境的影响却是不同的。Three neutral complexes of cobalt（Ⅱ） glycollates had been synthesized in the solution, in which cis- [Co2（Hgly）4（2,2＇-bpy）2] （3） （Hgly=glycolic acid, 2, 2＇-bpy=2,2＇-bipyridine） was characterized by X-ray structural analysis. It crystallized in the triclinic system, space group P2 1/c, a=1.003 7 （3） nm, b=2.022 6 （5） nm, c=1.660 1（4） nm, β=119.942（13）°, V=2.920 3 （13） nm^3. The interaction between bovine serum albumin （BSA） and complexes 1-3 had been investigated with fluorescence spectra at room temperature respectively. The fluorescence quenching was shown as static quenching for 2 and 3 based on fluorescence spectra at the different temperatures. The binding constants, binding sites and thermodynamic functions were calculated. The interaction sites and binding force of BSA with complexes 2 and 3 were proposed. These results showed that when the complexes containing phenanthroline and 2,2＇-bipyridine interacted with BSA respectively, the ligands of 1,10-phenanthroline and the 2,2＇-bipyridine both perturbed the microenvironment close to tryptophan residues of BSA, but the effects were different.

关 键 词：钴离子 乙醇酸 牛血清白蛋白 荧光光谱 

分 类 号：O614.4[理学—无机化学] O614.813[理学—化学]