多重PCR鉴别我国南疆3种常见蛉种  

Identification of Three Common Sandfies in Southern Xinjiang with Multiplex PCR

作　　者：周正斌[1] 张仪[1] 朱淮民[2] 施文琦[1] 金长发[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所、卫生部寄生虫病原与媒介生物学重点实验室、世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025 [2]第二军医大学病原生物教研室,上海200433

出　　处：《中国寄生虫学与寄生虫病杂志》2014年第3期185-187,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家十二五重大专项(No.2012ZX10004219);国家科技重大专项(No.2012ZX10004220,2008ZX10004011)~~

摘　　要：分别于我国南疆地区捕获长管白蛉64只、吴氏白蛉66只和亚历山大白蛉20只。本研究基于COⅠ基因序列差异,建立了鉴别我国南疆3种常见蛉种的多重PCR。结果显示,长管白蛉、吴氏白蛉和亚历山大白蛉的PCR产物分别为248、632和395 bp,且多重PCR结果与形态学鉴定结果完全一致。Based on the variable part of mtDNA CO I gene sequence, a multiplex PCR method was developed for the identification of the three common sandilies （Phlebotomus longiductus, Ph. wui, and Ph. alexandri） in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and could be used to identify the three Phlebotomus species. The PCR product of CO I gene from Ph. longiductus, Ph. wui and Ph. alexandri was 248, 632, and 395 bp, respectively.

关 键 词：多重PCR 长管白蛉 吴氏白蛉 亚历山大白蛉 中国 

分 类 号：R384.1[医药卫生—医学寄生虫学]