乙酰胺对毒鼠强染毒大鼠脑皮层氨基酸表达的影响  

Effects of acetamide at different doses on expression of amino acids in cerebral cortex of rats with acute tetramine poisoning

作　　者：王霞[1] 田英平[1] 姚冬奇[1] 高恒波[1] 苏建玲[1] 石汉文[1] 

机构地区：[1]河北医科大学第二医院急诊科,石家庄050000

出　　处：《中华劳动卫生职业病杂志》2014年第6期438-441,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的观察不同剂量乙酰胺对毒鼠强（TET）染毒大鼠脑皮层组织抑制性氨基酸γ-氨基丁酸（GABA）和兴奋性氨基酸谷氨酸（Glu）表达的影响。方法SPF级SD大鼠80只，随机分为5组（每组16只）：生理盐水对照组、二甲基亚砜水溶液（DMSO）对照组、TET染毒组、2．8g／kg/d乙酰胺、治疗组和5．6g／kg/d乙酰胺治疗组。染毒组和治疗组禁食后给予TET灌胃染毒，染毒后分别肌注生理盐水和不同剂量乙酰胺，连续5d，并分别于干预后3、12、48h和7d取材，用免疫组化方法分析各组大鼠脑颞叶皮层组织神经递质GABA和Glu的平均光密度（OD）值的变化。结果（1）GABA表达：TET染毒组OD值于12h开始升高，48h达峰值，7d恢复正常；2．8g／kg/d乙酰胺治疗组与TET染毒组相比OD值在12h升高不显著，48h时接近正常，低于染毒组，其变化更接近对照组；5．6g／kg/d乙酰胺治疗组OD值3h即明显升高，高于染毒组（P〈0．01），12h达高峰，48h提前恢复正常。（2）Glu表达：TET染毒组OD值3h较两对照组明显降低，12和48h逐渐上升，至7d恢复正常；2．8g／kg/d乙酰胺治疗组与TET染毒组OD值变化趋势大致相同，但在48h后有向对照组接近趋势；5．6g／kg/d乙酰胺治疗组与染毒组相比，3h时OD值高于染毒组（P〈0．01），12h时无明显差异，48h与7d时较各组明显下降（P〈0．01）。结论TET中毒给予大量乙酰胺治疗，对TET中毒的中枢损伤似有改善作用，而给予5．6g／kg/d乙酰胺治疗时，对神经介质表达影响复杂，治疗风险难于评估。Objective To investigate the effects of acetamide at different doses on the expression of inhibitory amino acids （gamma-aminobutyric acid, GABA） and excitatory amino acid （glutamate, Glu） in the cerebral cortex of rats with acute tetramine （TET） poisoning. Methods Eighty Sprague-Dawley rats （SPF） were randomly divided into five groups, with 16 rats in each group： saline control group, dimethyl sulfoxide （DMSO） control group, TET exposure group, high-dose （2.8 g/kg/d） acetamide treatment group, and super-high-dose （5.6 g/kg/d） acetamide treatment group. Rats in the exposure group and treatment groups were exposed to TET by intragastric administration after fasting, and were then intramuscularly injected with saline or different doses of acetamide in the following 5 days. The cortex of the temporal lobe was collected at 3 h, 12 h, 48 h, or 7 d after treatment. The expression levels of GABA and Glu in the cortex of the temporal lobe were determined by average optical density （OD） values in immunohistochemistry. Results 1） Expression of GABA： The OD value of GABA in TET exposure group started to increase at 12 h after treatment, reached the peak at 48 h, and decreased to the normal level at 7 d. In the high-dose acetamide treatment group, the increase in OD at 12 h was not so significant as that in the TET exposure group, OD value decreased to the normal level at 48 h and was lower than that in the exposure group, and the changes were more like those in the control groups. In the super-high-dose acetamide treatment group, OD value began to increase significantly at 3 h and was significantly higher than that in the TET exposure group （P〈0.01）, it reached the peak at 12 h, and was restored to the normal value at 48 h. 2） Expression of Glu： The OD value of Glu in TET exposure group at 3 h after treatment was significantly lower than those in the two control groups, it increased gradually from 12 h to 48 h, and recovered to the normal level at the 7th d. The changes in the

关 键 词：毒鼠强 中毒 乙酰胺 脑皮层 Γ-氨基丁酸 谷氨酸 

分 类 号：R595.4[医药卫生—内科学]