工作场所空气中2，4-二氯苯氧乙酸（2，4-滴）测定的高效液相色谱法  

Determination of 2, 4-dichlorophenoxyacetic acid in air of workplace by high-performance liquid chromatography

作　　者：温亚男[1] 付朝晖[2] 许建宁[1] 唐仕川[2] 王全凯[1] 李欢欢[1] 谢广云[1] 朱钰玲[1] 顾轶婷 谭枫[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]北京市劳动保护科学研究所北京市职业安全健康重点实验室

出　　处：《中华劳动卫生职业病杂志》2014年第6期458-459,共2页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：2013年职业卫生标准制（修）订项目（20130205）

摘　　要：目的建立工作场所空气中2，4-二氯苯氧乙酸（2，4-滴）的高效液相色谱测定法。方法空气中2，4-滴经超细玻璃纤维滤纸采集，甲醇洗脱，C18柱分离后，紫外检测器检测，保留时间定性，峰面积定量。结果方法线性范围为2-200μg／ml，洗脱效率为94．6％-95．9％，检出限（S/N=3）为0．034μg／ml（进样20μl洗脱液），定量下限为（S／N=10）为0．11μg／ml，最低检出浓度为0．011mg／m^3，最低定量浓度为0．037mg/m^3（以采集45L空气样品计）。结论该方法采样和样品处理方法简单，各项指标均可满足方法学要求，可用于工作场所空气中2，4-滴的测定。Objective To develop a method for determination of 2,4-dichlorophenoxyacetic acid （2,4- D） in the air of workplace by high-performance liquid chromatography. Methods 2, 4-D was collected by uhrafine glass filters, desorbed by methanol, separated by a Clg column, and detected by a UV detector. Identification and quantification of 2, 4-D were performed by retention time and peak areas, respectively. Results The linear range of the test was 2 -200 μg/ml; the elution efficiency was 94.6%-95.9%; the limit of detection （S/N=3） was 0.034 μg/ml （injection volume of 20 μl eluant）; the lower limit of quantification （S/N= 10） was 0.11 μg/ml; the minimum detectable concentration was 0.011 mg/m^3; the minimum quantifiable concentration was 0.037 mg/m^3 （with sampled air volume of 45 L）. Conclusion This method is convenient and simple in sample collection and preparation, and satisfies all methodological requirements. Therefore, this method is useful for the determination of 2, 4-D in the air of workplace.

关 键 词：2 4-二氯苯氧基乙酸 色谱法 高效液相 工作场所 

分 类 号：R134.4[医药卫生—劳动卫生] O657.72[医药卫生—公共卫生与预防医学]