干细胞丝素膜修复兔阴茎白膜缺损  

Silk fibroin scaffolds seeded with stem cells for repair of tunica albuginea defect in rabbits

作　　者：张亚[1] 周云[2] 连学刚[2] 苗小芬 王睿[3] 刘琳[1] 

机构地区：[1]苏州大学附属儿童医院儿科医学研究所,215003 [2]苏州大学附属儿童医院泌尿外科,215003 [3]苏州市中医医院病理科

出　　处：《中华创伤杂志》2014年第6期611-615,共5页Chinese Journal of Trauma

基　　金：江苏省临床医学科技专项资助项目（BL2012051）;苏州市社会发展科技资助项目（SYS201244）

摘　　要：目的研究脂肪间充质干细胞（adipose mesenchymal stem cells，ADMSCs）复合丝素膜在修复阴茎白膜缺损时纤维组织的重塑效果。方法选择雄性新西兰大白兔56只建立白膜缺损模型，按随机数字表法分为四组：A组为丝素膜修补组，B组为ADMSCs丝素膜修补组，C组为睾丸鞘膜修补组，D组为对照组，每组14只。术后6，12周行组织学HE染色、天狼星红染色、Hart染色和巨噬细胞免疫荧光染色检查，观察各组修复情况。结果术后12周HE染色显示，D组新生纤维组织排列紊乱，A、B、C组新生纤维组织排列整齐。天狼星红染色显示，Ⅰ型、Ⅲ型胶原纤维面积（以像素表示）A组为98780±4190，51177±5464；B组为94855±9010，50815±3895；C组为99860±6015，50948±6595；D组为79386±2237，85278±2645；其中A、B、C组胶原纤维以Ⅰ型为主，三组间差异无统计学意义，而D组Ⅰ型胶原纤维少于其他三组（P〈0．01），Ⅲ型胶原纤维多于其他三组（P〈0．01）。Hart染色显示，A、B、C和D组弹性纤维面积（以像素表示）分别为2805±90，2849±84，3068±485和1961±96，B、C组差异无统计学意义，A组显著少于B、C组（P〈0．01），D组显著少于其他各组（P〈0．01）。术后6周巨噬细胞阳性数A、B、C和D组分别为4．10±0，87，3．80±0，78，3．70±0．94和6．80±1．63，A、B、C组均显著低于D组（P〈0．01），A、B、C组差异无统计学意义。结论ADMSCs丝素膜在修复兔阴茎白膜缺损时能达到与自体组织修复基本同等的效果。Objective To investigate the effect of silk fibroin scaffolds seeded with adipose mesenchymal stem ceils （ADMSCs） in remodeling fiber for tunica albuginea defect in rabbits. Methods ,Fifty-six New Zealand rabbits were divided into 4 groups according to the random number table after a defeet was created in the tunica albuginea： Group A （the defect was repaired with silk fibroin） , Group B （with silk fibroin seeded with ADMSCs） , Group C （with autologous tunica vaginalis） and Group D （left unrepaired）, with 14 rabbits per group. Tunica albuginea sections were obtained for HE staining, Sirius red staining, Hart staining and immunofluorescence staining of macrophages at 6 and 12 weeks after surgery. Results At 12 weeks after surgery, HE staining revealed chaotically distributed new fiber in growth in Group D and orderly ingrowth in Groups A, B, and C. At 12 weeks after surgery, Sirius red staining revealed mean area of type I collagen fibers was greater than that of type Ⅲ in Groups A （98 780 ±4 190 vs 51 177 ±5 464）, B（94 855 ±9 010 vs 50 815 ±3 895）, and C（99 860 ±6 015 vs 50 948 ± 6 595 ）, but the difference in area of collagen fiber of the same type was insignificant among the three Groups. Moreover, less type I collagen fibers （79 386 ±2 237） and more type Ⅲ collagen fibers （85 278 ±2 645 ） were observed in Group D compared with other three Groups （ P 〈 0. 01 ）. At 12 weeks after surgery, Hart staining showed the mean area of elastic fibers in Groups A, B, C, and D was 2 805 ± 90, 2 849 ±84,3 068 ±485, and 1 961 ±96 respectively. There was no statistical difference between Groups B and C, but less amount of fibers was observed in Group A （P 〈 0. 01 ） and least amount was observed in Group D （P 〈0.01 ）. At 6 weeks after surgery, the number of infiltrating macrophages in Groups A, B, C and D was 4.10 ±0.87, 3.80 ±0.78, 3.70 ±0.94, and 6.80 ±1.63 respectively. There was no statistical difference in the number of macrophage infi

关 键 词：间质干细胞 丝素蛋白 白膜缺损 

分 类 号：R318.08[医药卫生—生物医学工程]