水红花子对LPS诱导流产小鼠子宫巨噬细胞的抑制效应及其保胎作用  

Inhibitory effects and tocolysis role of fructus polygoni orientalis on macrophages in uterine of abortion mice induced by LPS

作　　者：钱志英[1] 刘亚洁[1] 崔艳品 谢李宁 张顺利[1] 

机构地区：[1]河南师范大学生命科学学院省部共建细胞分化调控国家重点实验室,新乡453007

出　　处：《中国免疫学杂志》2014年第6期763-767,共5页Chinese Journal of Immunology

基　　金：河南省科技攻关项目(No.0524420040);河南省动物学省级重点学科基金资助

摘　　要：目的:探讨水红花子对细菌脂多糖(LPS)诱导流产小鼠的免疫抑制及其保胎作用。方法:昆明种小鼠55只,随机分为对照组(A组,10只)和实验组。实验组又分为细菌脂多糖(LPS)处理组(B组),水红花子处理组(C组),水红花子及LPS双处理组(D组),每组各15只。观察妊娠结果,用酶组织化学、免疫组化和ELISA方法分别检测非特异性酯酶阳性(α-NAE+)、CD14+和CD204+巨噬细胞、TNF-α的变化。结果:B组小鼠流产率和胚胎吸收率均为100%(P<0.01);而D组流产率降低为13.33%,胚胎吸收率降至10.39%。与A组相比,在B组,α-NAE+、CD14+巨噬细胞数量和阳性面积,以及TNF-α含量均显著增加;在D组,环肌外侧极显著增多(P<0.01),但环肌内侧、功能层均接近正常水平。与A、B组相比,C、D组CD204+巨噬细胞数量和阳性面积均显著增多。结论:通过调节子宫巨噬细胞的表型、活性和TNF-α的分泌,可能是水红花子对抗LPS所致流产效应的机制之一。Objective：To explore the immunosuppression effects and tocolysis of fructus polygoni orientalis on abortion mice induced by LPS.Methods：Mice of Kunming （55 mice） were divided into control group （group A ,10 mice） and experimental group. Experimental group were divided into group B （ intraperitoneal injection LPS ） , group C （ ingestion fructus polygoni orientalis ） , and group D （ingestion fructus polygoni orientalis and intraperitoneal injection LPS ）,each group of 15 mice.Then the pregnancy results were observed ,the positive of α-NAE＋and the varity of CD 14＋and CD204＋macrophages ,TNF-αin the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA.Results：The abortion rate and the embryo resorbing rate were all 100%（ P〈0.01 ） in group B.But there was the decreased abortion rate of 13.33% in group D .The embryo resorbing rate decreased to 10.39%.The number and positive cell area of α-NAE＋and CD14＋macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A （ P〈0.01 ） .These effects outside of myometrium of group D were remarkably increased comparing with group A （P〈0.01）,but there was no distinct difference in the inside of myometrium and function layer.The number and positive cell area of CD204＋macrophages in group C and D was greatly increased comparing with group A and B （ P〈0.01 ） .The TNF-αcontents in the uteri of mice in group B were greatly increased comparing with group A （P〈0.01）,but the positive cell area of CD14,CD204 were close to normal levels in group D.Conclusion：The effect of miscarriage induced by LPS is antagonized by fructus polygoni orientalis through inhibiting the phenotype ,activity and function characteristics of macrophages in the uteri of gravidity mice.

关 键 词：水红花子 巨噬细胞 CD14+ CD204+ TNF-α 流产 小鼠 

分 类 号：R967[医药卫生—药理学]