食蟹猴实验感染HBV的血清学反应  

Serological response infected with on cynomolgus monkeys

作　　者：王振常[1] 梁增文[2] 冷静[3] 韦毅 黄晶晶[1] 陈松林[1] 

机构地区：[1]广西中医药大学第一附属医院,南宁530023 [2]广西壮族自治区人民医院消化内科,南宁530021 [3]广西中医药大学微生物与免疫学教研室,南京530001 [4]防城港常春生物技术开发有限公司,防城港538001

出　　处：《中国免疫学杂志》2014年第6期814-816,共3页Chinese Journal of Immunology

基　　金：广西科技基础平台建设项目(12-97-22);广西高校科技创新平台(桂教科研[2013]8号);广西教育厅重点学科实验室项目

摘　　要：目的：研究食蟹猴感染乙型肝炎病毒（ HBV）后的血清学反应。方法：实验室内人工繁育的1～3日龄或成年食蟹猴观察1个月经病毒学筛选后证实为健康动物后各随机分为对照组、感染组，感染组接种HBV携带者血清0．5 ml（ HBV-DNA≥108拷贝）单只笼养，各组从接种后1～12周每日观察行为变化，每1周取血样检测HBV-M、HBV-DNA、肝功能及对于HBsAg阳性食蟹猴在B超引导下取肝组织常规HE染色检测肝组织炎症程度。结果：成年猴接种后未引发HBsAg阳性反应，有3只幼年猴出现HBsAg、HBcAb及2只出现HBV-DNA反应，ALT在攻毒出现HBsAg阳性后1周开始升高，1个月后达到高峰，其值为180 U/L，以后渐降，持续1个月后接近正常。 AST一周后高于正常参考值呈低平曲线，高峰较ALT迟后1个月， HBsAg阳性食蟹猴HE染色可见部分肝组织呈轻微肝炎病变。结论：攻毒后HBV-M、HBV-DNA、ALT、AST及肝组织病理改变提示HBV感染后能产生应答及肝细胞炎症反应。Objective：To study the serological reactions in cynomolgus monkeys infected with hepatitis B virus （ HBV）. Methods：To select 1 to 3 days old or adult healthy cynomolgus monkeys by artificial breeding to observe the virology screening in laboratory a month to confirmed healthy animals ,randomly divided into control group and infection group .Infection group vaccination serum HBV carriers 0.5 ml （HBV-DNA≥108 copies） single cages,observe each group behavioral changes daily after inoculation 1 to 12 weeks, each week to confirmed the degree of liver inflammation through the HBV-M, HBV-DNA, liver function and on the B-guided, liver tissue inflammation by routine HE staining .Results： Adult monkeys did not induce positive reaction after vaccination , there were three young monkeys appear HBsAg , HBcAb and 2 appear HBV-DNA reaction, ALT poison attack occurred in HBsAg-positive began to increase after one week , one month after the peak , which was 180 U/L, after gradually decreased , continuing a month later near normal .AST higher than a week after the normal reference values were flat curve , representing the peak ALT after a month later, HBsAg positive cynomolgus monkeys HE staining showed mild hepatitis partial liver tissue lesions .Conclusion：HBV-M, HBV-DNA, ALT, AST and liver histopathology after HBV infection have changed , this result showing that it＇s produce inflammation and induction the response of immune .

关 键 词：食蟹猴 肝炎病毒 乙型 动物模型 

分 类 号：R245.8[医药卫生—针灸推拿学]