去势手术方式对小鼠卵巢组织自体皮下移植效果的影响  

Influence of castration methods on the subcutaneous auto- transplantation of mouse ovaria

作　　者：李宇彬[1] 麦庆云[1] 李涛[1] 周灿权[1] 

机构地区：[1]中山大学附属第一医院生殖中心,广州510080

出　　处：《广东医学》2014年第11期1647-1649,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81100470;81270750);中山大学青年教师培育计划项目(编号:12ykpy24)

摘　　要：目的探讨两种去势手术方式对联合免疫缺陷(SCID)小鼠卵巢自体皮下移植效果的影响。方法按照手术方式不同将SCID小鼠分为3组:对照组、腹式组以及背式组,每组10只。腹式组和背式组术后分别进行腹壁皮下及背部皮下自体卵巢组织移植。记录两组手术所用时间及术后小鼠存活率。2周后与对照组(无手术处理)都进行了促排卵。其后回收卵巢组织进行卵泡存活率分析。结果腹式组有3只小鼠术后死亡,对照组及背式组均无小鼠死亡。腹式组较背式组手术所用时间长,分别为(18.6±2.7)min和(12.5±3.1)min,差异有统计学意义(P<0.05)。腹式组和背式组术后卵巢组织卵泡存活数较对照组减少(均P<0.05),但两组间差异无统计学意义(P>0.05)。结论小鼠背式去势手术并自体卵巢组织皮下移植相比腹式手术更加快捷简单,两术式可获得相似的移植后卵泡生存率。Objective To investigate the influence of two castration methods on subcutaneous auto - transplanta-tion of mouse ovarian tissue in SCID mice. Methods According to the operation methods,the mice were divided into three groups：control group,abdominal castration group and backside castration group（n = 10）. The operation time and mouse survival rates were recorded after operation. Ovarian stimulation was carried out in all 3 groups 2 weeks later,after which follicle survival rate was recorded. Results There were 3 deaths after operations in the abdominal castration group, while no death was revealed in the other two groups. Significantly lengthened operation time was observed in abdominal castration group than backside castration group[（18. 6 ± 2. 7）min vs（12. 5 ± 3. 1）min,P 〈 0. 05]. The follicle surviv-al rates in the two castration groups were significantly less than control group（P 〈 0. 05）,while there was no significant difference between the two castration groups（P 〉 0. 05）. Conclusion Backside castration combined subcutaneous auto- transplantation of ovarian tissue was simpler than the abdominal one,with similar follicle survival rates.

关 键 词：卵巢组织 自体异位移植 去势 

分 类 号：R713.6[医药卫生—妇产科学]