Ficoll分离液在Sephadex G-100微柱凝胶血型卡中的应用  

Development of Ficoll-Sephadex G-100 microcolumn technique for blood group serological detection

作　　者：黄文彩[1] 梁鉴波[2] 付文金[1] 陈璇[1] 余洁玲[1] 方爱娟[1] 李金洳 

机构地区：[1]广东医学院附属东莞市厚街医院,523940 [2]中山大学附属第一医院检验科,510080

出　　处：《国际医药卫生导报》2014年第14期2145-2148,共4页International Medicine and Health Guidance News

摘　　要：目的 探讨Sephadex G-100微柱凝胶血型卡加入Ficoll分离液后的性能和应用.方法 在Sephadex G-100微柱凝胶血型卡中加入Ficoll分离液,制作成Ficoll分离液-微柱凝胶血型卡,对200例标本进行ABO和Rh（D）血型测定,并与进口的强生血型卡和Sephadex G-100血型卡法进行比对.分别用37℃孵育强生血型卡、45℃孵育强生血型卡和45℃孵育Ficoll分离液-微柱凝胶血型卡三种方法对6例低、中、高不同效价的冷凝集素标本进行ABO和Rh （D）血型测定,同时进行反定型血型检测.分别用强生血型卡、Sephadex G-100血型卡和Ficoll分离液-微柱凝胶血型卡对26例溶血标本进行ABO和Rh （D）血型测定.结果 自制Ficoll分离液-微柱凝胶血型卡检测200例标本的血型结果,A型55例,B型46例,O型92例,AB型7例,Rh （D）均为阳性,与强生血型卡和Sephadex G-100微柱凝胶血型卡结果相同.自制血型卡检测抗D抗体平均滴度为1：256,与Diana凝胶卡灵敏度相同,明显优于传统试管法（P＜0.05）.45℃孵育Ficoll分离液-微柱凝胶血型卡检测6例冷凝集素标本血型结果正确,正反定型一致,37℃和45℃孵育强生血型卡检测冷凝集素标本血型分别有3例和2例结果不正确,造成假阳性.Ficoll分离液-微柱凝胶血型卡检测26例溶血标本血型结果正确,强生血型卡和Sephadex G-100血型卡检测溶血标本血型分别有6例和10例结果不正确.结论 研制Ficoll分离液-Sephadex G-100微柱凝胶血型卡适用于血型鉴定.对于冷凝集和溶血标本血型鉴定有明显优势.该卡具有准确、灵敏、特异、试剂制备工艺简单、成本低等优点,具有较好的推广应用和开发价值.Objective To explore the performance and application of Ficoll-Sephadex G-100 miacrocolumn technique for blood group serology.Methods Ficoll-Sephadex G-100 microcolumn was made of Sephadex G-100 by joining Ficoll liquid separation.200 specimens of ABO and Rh （D） blood type were identified by Ficoll-Sephadex G-100 microcolumn and BioVue.The difference between the two methods was compared.Six specimens of cold agglutinin syndrome in different titers were detected by BioVue incubating at 37 ℃ and 45 ℃ respectively,and by Ficoll-Sephadex G-100 microcolumn incubating at 45 ℃.26 hemolysis samples were selected for ABO and Rh （D） blood group identification by Ficoll-Sephadex G-100 microcolumn,BioVue,and Sephadex G-100 microcolumn respectively.Results The results of the 200 samples tested by Home-made blood group cards were consistent with those by BioVue and Sephadex G-100 microcolumn.The mean of anti-D antibody titers in the Home-made cards was 1： 256,which was consistent with that in BioVue.Home-made blood group cards were significant better than test tube method （P 〈 0.05）.The results of 6 cold agglutination samples were all correct for Home-made blood group cards,but 3 and 2 incorrect results incubated by BioVue at 37℃ and 45℃,respectively.The results of 26 hemolysis samples were all correct for Home-made blood group cards,6 and 10 incorrect results for BioVue Sephadex G-100 microcolumn.Conclusions Ficoll-Sephadex G-100 microcolumn has nice coincidence,sensitivity and repetitiveness and is a cheap and easy method.It is suitable for blood group typing.

关 键 词：Ficoll分离液 SEPHADEX G-100微柱凝胶血型卡 血型鉴定 冷凝集 溶血 

分 类 号：R446.6[医药卫生—诊断学]