HPSEC法检测注射用双黄连(冻干)中的高分子量物质  

Determination of High Molecular Weight Substances in Shuanghuanglian Injection(freeze-drying) by HPSEC

作　　者：裴福成 苏德龙 李文春 任桂萍[2] 

机构地区：[1]哈药集团中药二厂,黑龙江哈尔滨150078 [2]东北农业大学,黑龙江哈尔滨150030

出　　处：《中医药学报》2014年第3期52-54,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家科技重大专项子课题重大新药创制(2011ZX09201-201-15);黑龙江省科技计划项目(GA09C308);哈尔滨市科技攻关计划项目(2010AA3CS025)

摘　　要：目的:建立注射用双黄连(冻干)中高分子量物质的检测方法。方法:采用凝胶色谱柱(TSKG2000SWxl,5μm,7.8mm×300mm);以三氟乙酸-乙腈-水(0.05:20:80)为流动相;蒸发光散射检测器检测;流速为0.7mL/min。结果:注射用双黄连(冻干)中未检出高分子量物质。结论:所建立的方法简便、准确、灵敏度好,适合检测注射用双黄连(冻干)中的高分子量物质。Objective：To establish a method for the determination of high molecular weight substances in Shuanghuanglian injection （freeze-drying）.Methods：TSK-G2000 SWxl（5μm,7.8mm ×300mm）column was used.The mobile phase was a mixed solution of trifluoracetic -CH3 CN-Water（0.05：20：80） with the flow rate of 0.7mL/min.ELSD detector was used .Results：The high molecular weight substances were not detected in Shuanghuanglian injection （ freeze-dr-ying）.Conclusion：The method is simple,accurate,sensitive and reliable for the determination of high molecular weight substances in Shuanghuanglian injection （freeze-drying）.

关 键 词：注射用双黄连(冻干) 高分子量物质 分子排阻色谱法 

分 类 号：R284.1[医药卫生—中药学]