地黄饮子对快速老化小鼠SAMP8线粒体通路细胞凋亡的影响  

Effect of Di Huang Yin Zi on Apoptosis of Mitochondrial Pathway in Hippocampus of Senescence Accelerated Mouse Prone 8

作　　者：姚辛敏[1] 王琪[1] 周妍妍[1] 袁晓霞[2,3] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]新疆医科大学,新疆乌鲁木齐830011 [3]新疆名医名方与特色方剂学实验室,新疆乌鲁木齐830011

出　　处：《中医药学报》2014年第3期138-140,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金青年基金项目(No.81102546);新疆名医名方与特色方剂学实验室开放课题资助项目(No.XJDX0910-2012-6)

摘　　要：目的:研究地黄饮子对快速老化小鼠P8线粒体通路细胞凋亡的影响,探讨其治疗AD的药效及作用机制。方法:运用RT-PCR检测地黄饮子对SAMP8小鼠Caspase-3、Caspase-9、Apaf-1基因mRNA表达的影响,观察小鼠细胞凋亡情况。结果:地黄饮子能下调海马Caspase-3mRNA、Caspase-9mRNA、Apaf-1mRNA的表达,且均有统计学意义。结论:地黄饮子可通过对细胞凋亡的线粒体途径的影响,抑制促凋亡基因,减少神经元的丢失,从而改善学习记忆能力。Objective：To investigate the pharmacodynamic mechanisms of Di Huang Yin Zi （ DHYZ） on apoptosis of mito-chondrial pathway of senescence accelerated mouse prone 8.Methods：RT-PCR was used to detect the expression of Apaf-1mRNA,Caspase-9mRNA and Caspase-3mRNA.Results：Compared with model group ,DHYZ could decrease the expression of Apaf -1 mRNA, Caspase -9mRNA, Caspase -3mRNA in the hippocampus of SAMP8 mice,which was statistically significant .Conclusion：The apoptotic inhibition of DHYZ for SAMP 8 mice may be through repairing mi-tochondrial damage and decreasing the expression of pro -apoptotic factors to reduce the loss of neurons .

关 键 词：地黄饮子 SAMP8 细胞凋亡 线粒体通路 

分 类 号：R285.5[医药卫生—中药学]