逍遥散抗大鼠肝纤维化活性部位的筛选  

Preliminary Screening of Anti-hepatic Fibrosis Fractions from Xiaoyao San

作　　者：王业秋[1] 任燕冬[1] 刘海洋[1] 张宁[1] 陈巧云[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中医药学报》2014年第3期141-143,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省教育厅科学技术研究面上项目(11551465)

摘　　要：目的:初步筛选出逍遥散抗大鼠肝纤维化的活性部位。方法:建立大鼠肝纤维化模型,观察逍遥散提取物不同洗脱部位对肝纤维化大鼠ALT、AST、γ-GT、TBIL/HA、LN、PCⅢ、Ⅳ-C的影响。结果:20%醇洗脱组的ALT、AST、γ-GT,50%醇洗脱组的ALT、AST、γ-GT、TBIL、HA、LN、PCⅢ、Ⅳ-C,80%醇洗脱组的ALT、γ-GT、HA下降明显,与模型组相比,差异具有统计学意义(P<0.05或P<0.01)。结论:50%醇洗脱组的效果优于水洗组、20%、80%醇洗脱组,此部位为逍遥散抗大鼠肝纤维化的活性部位。Objective：To estimate the anti-hepatic fibrosis activity of the extracts of Xiaoyao San in rats .Methods：Liver fibrosis model in rats was established by intraperitoneal injection of 10%CCL4 -oliver oil.Observe the effect of differ-ent extract fractions from Xiaoyao San by testing the indexes of hepatic function （ ALT, AST,γ-GT, TBIL） and the se-rum four items of hepatic fibrosis （HA, LN, PC-Ⅲ, Ⅳ-C）.Results：The serum levels of ALT, AST, γ-GT in 20%ethanol extract fraction, the serum levels of ALT, AS,γ-GT, TBIL, HA, LN, PCⅢ, Ⅳ-C in 50%ethanol extract fraction and the serum levels of ALT ,γ-GT, HA in 80%ethanol extract fraction decreased significantly com-pared with the model group （ P〈0 .05 or P〈0 .01 ） .Conclusion： The effect in 50% ethanol extract fraction is better than 20%and 80%ethanol extract fractions and the anti -hepatic fibrosis active fractions of Xiaoyao San are 50%etha-nol extract fraction .

关 键 词：逍遥散 大鼠 肝纤维化 活性部位 

分 类 号：R285.5[医药卫生—中药学]