基于OsGS5的玉米同源基因ZmGS5的克隆  

Cloning of homologous gene ZmGS5 in maize based on OsGS5

作　　者：李彩云[1] 孔曼丽[1] 孙清鹏[1] 卢敏[1] 王维香[1] 潘金豹[1] 韩俊[1] 

机构地区：[1]农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京102206

出　　处：《北京农学院学报》2014年第3期14-17,共4页Journal of Beijing University of Agriculture

基　　金：北京市教育委员会科研计划面上项目(KM201110020002);国家自然科学基金(31301312)

摘　　要：高产是玉米育种的重要目标,籽粒大小是决定籽粒产量的重要因子。OsGS5是水稻中已克隆的控制籽粒长度和千粒重的主效基因,编码丝氨酸羧肽酶。本研究利用OsGS5基因编码序列比对玉米同源EST序列,并对EST序列进行拼接,根据拼接后的EST序列设计3′-RACE基因特异引物,利用同源克隆的方法克隆出玉米同源基因的3′端基因片段,经测序发现该基因片段长度为981bp,功能注释后发现该基因编码丝氨酸羧肽酶,与水稻OsGS5基因编码蛋白具有高度同源性,将该基因暂时命名为ZmGS5。High yield is an important goal of the maize breeding program, grain size is a major determinative factor of cereal grain yield. OsGS5, encoding a serine carboxypeptidase, is a key gene for controlling grain length and 1000-grain weight in rice, which was isolated by the map-based cloning strategy. In this study, the OsGS5 sequence was used as a query sequence to search for maize homologous EST sequences, the targeted EST sequences were assembled into a consensus sequence, and designed the gene＇s specific primers for 3r-RACE. Homology cloning was conducted to isolate the 3＇region of orthologous gene from maize genome, the sequencing result revealed that thehomologous fragment was 981hp in length,the result of function annotation indicated that the orthologous gene also encoded a serine carboxypeptidase with high similarity to OsGS5 in amino acid sequence, the gene named ZrnGS5 temporarily.

关 键 词：玉米 ZmGS5 OsGS5 丝氨酸羧肽酶 克隆 

分 类 号：S513[农业科学—作物学]