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作 者:孙朦朦[1] 黄亚冬 孙学亮[1] 郭永军[1] 陈成勋[1] 邢克智[1]
机构地区:[1]天津农学院水产学院天津市水产生态及养殖重点实验室,天津300384 [2]天津海升水产养殖有限公司,天津300272
出 处:《天津农学院学报》2014年第2期13-17,共5页Journal of Tianjin Agricultural University
基 金:天津市星火计划项目"黄金鲫良种繁育及健康养殖技术示范"(09ZHXHNC05100);国家星火计划项目"黄金鲫工厂化育苗及健康养殖技术示范"(2010GA610007);天津市农业科技示范推广项目"黄金鲫良种繁育及健康养殖技术推广"(201001010);国家级科技计划项目"水产品健康养殖;加工与质量安全控制产业化示范"(2013GA610003)
摘 要:为研究腹腔注射Cd2+对黄金鲫血液生化指标的影响,试验将300尾健康黄金鲫随机分为4组,分别为对照K组、A组、B组和C组,其中Cd2+的注射浓度依次为0、1.25、2.50、3.75 mg/kg,测定注射后0、1、3、5、7 d时的血清超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)和丙二醛(MDA)的变化。结果表明,鱼体血液中各指标的变化与注射镉元素密切相关。与对照组相比,A组血清中SOD活力在前3天显著升高(P<0.05),在第5、7天时差异不显著(P>0.05),B组和C组中SOD活力在整个试验期间显著降低(P<0.05);A组、B组和C组血清中CAT、ACP活性和MDA含量与对照相比均显著增加(P<0.05);A组AKP活性在第1天时与对照组无显著差异,之后显著降低,B组和C组均显著低于对照组(P<0.05)。It is aimed to investigate the effects of intraperitoneal injection of Cd^2 + on serum biochemical indictors of golden carp. 300 healthy golden carps were randomly divided into 4 groups with 3 replicates per group, namely the control group, A group, B group, C group and Cd2 ~ concentration of these groups were 0, 1.25, 2.50 and 3.75 mg/kg in turn, serum superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT), malondialdehyde (MDA) contents among these different groups were determined at 0, 1, 3, 5, 7 d after injection respectively. The results show as follows. The changes of serum indicators were closely related with injecting cadmium elements. Compared with the control group, serum SOD activity in A group was significantly higher in the first three days (P〈0.05) , and there was no significant difference at 5, 7 days, while the serum SOD activity in group B and group C during the whole trial period was significantly lower than that of the control group (P〈0.05) . The serum CAT activity, ACP activity and MDA content in group A, group B and group C were significantly higher than those of the control group (P〈0.05). AKP activity in A group compared with the control group had no significant difference in the first day, and then decreased significantly, AKP activity in group B and group C were significantly lower than that of the control group (P〈0.05) .
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