大豆高效体细胞胚诱导和增殖方法的研究  

Research to the Effective Methods of Soybean Somatic Embryo Induction and Proliferation

作　　者：程琳静[1] 闫军辉[1] 钟云鹏[1] 姚陆铭[1] 王彪[1] 卢欣欣[1] 武天龙[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《大豆科学》2014年第3期305-310,共6页Soybean Science

基　　金：国家转基因重大专项(2013ZX08004-003);引进国际先进农业科学技术计划"948计划"(2011-G1(2)-16);上海市重点学科建设基金(B209)

摘　　要：以10个不同基因型的大豆幼胚为材料进行组织培养,研究不同播种季节和基因型对体细胞胚诱导率的影响,以及继代培养基状态和蔗糖含量对体细胞胚增殖的影响。结果表明:(1)同一基因型大豆幼胚,不同播种季节对体细胞胚诱导率的影响表现为春季>温室>秋季>夏季;(2)在同一培养和诱导条件下,体细胞胚诱导率较高的基因型是垦丰23,北4217,绥农30和绥农28;(3)液体的继代培养基相对固态,更有利于体细胞胚增殖;(4)继代培养基中高浓度蔗糖(60 g·L-1)有利于体细胞胚的增殖。With 10 genotypes of soybean immature embryos obtained from different seasons, the effects of different planting seasons and genotypes on somatic embryogenesis induction were primarily studied. And in order to optimize the regeneration system of soybean somatic embryos, the impact of liquid and solid subculture medium and the concentration of sucrose on the proliferation of somatic embryos were further researched. The result showed that （ 1 ） For the same genotype of soybean immature embryos, the effect of different planting conditions on somatic embryogenesis induction rate was spring 〉 greenhouse 〉 autumn 〉 summer. （2）In the same culture condition, the genotypes which possessed the higher embryo induction rate were Kenfeng 23, North 4217, Suinong 28 and Suinong 30. （3）Compared to solid culture,liquid culture was favor of the proliferation of somatic embryos. （4）Subculture medium containing high concentration of sucrose（60 g· L^-1）was conducive to the proliferation of somatic embryos.

关 键 词：基因型 体细胞胚 诱导率 继代培养基 

分 类 号：S565.1[农业科学—作物学]