大豆GmPLP1启动子的克隆及功能分析  

Cloning and Functional Analysis of a Promoter of GmPLP1 Gene in Soybean

作　　者：李永光[1] 张宇航[1] 罗秋兰[1] 谷翰卿[1] 李文滨[1] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,东北农业大学农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2014年第3期311-314,共4页Soybean Science

基　　金：抗逆转基因大豆新品种培育(2014ZX08004-002);国家自然科学基金(31201228)

摘　　要：本研究利用染色体步移法从大豆栽培品种东农L13基因组中分离得到了GmPLP1基因1.5 kb的启动子片段,利用PLACE和PlantCARE在线启动子预测工具发现了107个调控元件。将该片段定向替换载体pBI121的CaMV35S组成型启动子,构建表达载体pBI121-pGmPLP1,驱动下游报告基因GUS基因表达,并通过组织化学染色和GUS定量分析对预测结果进行验证,结果显示GmPLP1受黑暗、蓝光、低温、GA3和ABA诱导表达。To reveal the molecular mechanism underlying the expression of soybean PLP1 （ GmPLP1 ） gene that contains PAS/LOV domain,its 5＇ regulatory region was analyzed. The1. 5 kb fragment upstream of the GmPLP1 gene was isolated from soybean cuhivar ‘ Dongnong L13＇ by genome walking. There were 107 cis-acting elements in the promoter predicted by online software Plant CARE and PLACE. the promoter fragment deletions were inserted into the 5＇ region of the gus. A reporter gene in vector pBI121 （replacing the 35S promoter）. In order to determine the promoter characteristic of GmPLP1. The constructs were transferred into Nicotiana tabacum and Glycine max. The GUS activity was up-regulated under dark, blue light, gibberellin A3 and abscisic acid driven by GmPLP1 promoter.

关 键 词：GmPLP1启动子 GUS基因 组织化学染色 顺式调控元件 

分 类 号：S565.1[农业科学—作物学]