紫杉醇对大鼠血管平滑肌细胞G_1-S检查点作用的研究  

Study on the effect of paclitaxel on G1-S checkpoint of rat vascular smooth muscle cell

作　　者：静亮 彭希[1] 谢敏杰[1] 喻志源[1] 吕家高[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2014年第3期193-195,共3页Journal of Critical Care In Internal Medicine

基　　金：国家自然科学基金资助项目(No:81030021)

摘　　要：目的:探讨紫杉醇对大鼠血管平滑肌细胞(VSMC)和血管内皮细胞(VEC)G1-S检查点的作用。方法:将大鼠VSMC与VEC进行原代培养,50 nmol/L紫杉醇干预72 h后进行免疫荧光染色和蛋白印迹法检测。结果:同VEC相比,紫杉醇干预的VSMC中BrdU表达明显增加(均P<0.05)。紫杉醇干预的VSMC中cyclin D1、P27表达高于VEC,P21表达较低(均P<0.05)。结论:紫杉醇干预的VSMC同VEC相比,在增殖率与细胞周期调节因子表达方面具有明显差异,紫杉醇对VSMC G1-S检查点有特异性调节作用。Objective： To explore the effects of paclitaxel on G1 -S checkpoint of vascular smooth muscle cell （VSMC） and vascular endothelial cell （VEC） of rats. Methods： VSMC and VEC of rats underwent primary culture and were intervented by 50 nmol/L paclitaxel for 72 hours and then evaluated by immunohistochemistry and western blotting. Results： Compared with VEC, the content of BrdU was increased in paclitaxel-treated VSMC （P 〈 0.05）, paclitaxel-treated VSMC could express more cyclin D1, P27 and fewer P21 （P 〈 0. 05 ）. Conclusions ： Paclitaxel-treated VSMC shows significant differences in the expressions of cell cycle regulators and proliferation rates as compared with those of VEC, thus suggests the specific G1 -S checkpoint regulation effect of paclitaxel on VSMC.

关 键 词：血管平滑肌细胞 血管内皮细胞 紫杉醇 G1-S检查点 

分 类 号：R543.3[医药卫生—心血管疾病]