广金钱草水提取液对肾草酸钙结石模型大鼠氧化应激的影响研究  

Effects of aqueous extract from Desmodium styracifolium （Osb.） Merr.on oxidative stress of renal calcium oxalate rat models

作　　者：向松涛[1] 甘澍[1] 周建甫[1] 荣向路[2] 汪茜[1] 赵钟祥[2] 苏俊芳[3] 王树声[1] 

机构地区：[1] 广州中医药大学附属广东省中医院泌尿外科, 广州510120 [2] 广州中医药大学中药学院 [3] 广州中医药大学基础医学院

出　　处：《中华泌尿外科杂志》2014年第6期465-468,共4页Chinese Journal of Urology

基　　金：国家中医药管理局科学技术研究专项（04-05ZP47）;广东省科技计划项目（20088030301363,20118061300077）;广州中医药大学科研创新基金项目（2004）

摘　　要：目的 探讨广金钱草水提取液对肾草酸钙结石模型大鼠氧化应激的影响. 方法 采用乙二醇饮水和氯化铵灌胃法诱导建立大鼠肾草酸钙结石模型,将50只8周龄Wistar健康雄性大鼠按完全随机设计法随机分为5组（n=10）：空白组（A组）、模型组（B组）、广金钱草低剂量组（C组）、广金钱草中剂量组（D组）和广金钱草高剂量组（E组）,其中A组采用自来水饮水＋生理盐水2 ml/d灌胃,B组采用1％乙二醇饮水＋2％氯化铵2 ml/d灌胃,C、D、E组在B组基础上分别采用0.6、1.2、2.4 g/ml广金钱草水提取液2 ml/d灌胃28 d.各组大鼠在相同条件下饲养28 d后收集血液及双肾标本,左肾组织做HE染色石蜡切片,光镜观察草酸钙结晶情况;测定血液和右肾匀浆组织中丙二醛、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）含量等了解氧化应激情况. 结果 肾组织草酸钙结晶方面,与A组（0±0）比较,B组（7.1±2.9）结晶评分显著增高（P＜0.01）,C组（1.6±1.4）、D组（0.4±0.7）和E组（1.1±1.1）结晶评分较B组显著降低（P＜0.01）,其中D组降低最显著.氧化应激指标方面,与A组比较,B组大鼠血、肾组织丙二醛浓度显著升高（P＜0.01）,使用广金钱草水提取液（C组、D组、E组）干预可减少血、肾组织丙二醛水平（P＜0.01）.与A组比较,B组大鼠血、肾组织SOD、GSH-Px、CAT的浓度显著降低（P＜0.01）,使用广金钱草水提取液各剂量组（C组、D组、E组）干预可以显著增加肾匀浆SOD水平（P＜0.01）,其中D组尚可显著增加血SOD、GSH-Px的水平（P＜0.05）,但广金钱草水提取液各剂量组对血CAT及肾匀浆GSH-Px、CAT无显著影响（P＞0.05）. 结论 中剂量广金钱草水提取液在肾草酸钙结石模型大鼠中可明显抑制晶体形成,并有一定的抗氧化作用.Objective To investigate the effects of aqueous extract from Desmodium styracifolium （Osb.） Merr.（Ds） on oxidative stress of renal calcium oxalate （CaOx） rat models.Methods The models of renal CaOx were established with ethylene glycol in drinking water and ammonium chloride by gavage.The 50 adult healthy male Wistar strain rats were randomly divided into 5 groups （10 of each）,i.e.,normal control group （group A）,calcium oxalate model group （group B）,low-dose Ds extract-treated group （group C）,moderate-dose Ds extract-treated group （group D） and high-dose Ds extract-treated group （group E）.Group A was established with drinking water and physiological saline by gavage （2 ml per day）,group B was established with 1% ethylene glycol in drinking water and 2% ammonium chloride by gavage （2 ml per day）,group C,D and E were established with aqueous extract from Ds （0.6,1.2 and 2.4 g/ml,respectively） by gavage on the basis of group B （2 ml per day）.After feeding for 28 days,blood and renal samples were collected.The presence of CaOx crystals was scored in left kidneys under hematoxylin and eosin staining,and the levels of malondialdehyde （MDA）,superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px） and catalase （CAT） in the blood and right kidney homogenate were measured as indicators of oxidative stress.Results For the formation of CaOx crystals,the scores of group B were significantly higher than group A （P〈0.01）,the scores of group C,D and E were significantly lower than group B （P〈0.01）,especially the group D showed the best effect.For the parameters of oxidative stress,compared with group A,the concentration of MDA in blood and kidney tissues of group B significantly increased （P〈0.01）,which significantly decreased （P〈0.01） after the intervention of Ds （group C,D and E）.Compared with group A,the content of SOD,CAT and GSH-Px in group B significantly decreased （P〈0.01）,the SOD level in renal homogenate significantly

关 键 词：草酸钙结石 广金钱草 氧化应激 活性氧 

分 类 号：R285.5[医药卫生—中药学]