肠道病毒71型手足口病病毒3种检测技术比较研究  

Comparative study of three techniques for the detection of EV71 virus

作　　者：慕毅敏[1] 代岩石[1] 孙伟[1] 刘笑竹[1] 

机构地区：[1]四平市疾病预防控制中心微生物检验科,吉林四平136000

出　　处：《中国卫生工程学》2014年第3期232-234,共3页Chinese Journal of Public Health Engineering

摘　　要：目的探讨不同方法在肠道病毒71型(EV71)检测中的效果对比分析,方便对EV71做出及时而有效的诊断,为该病快速鉴别诊断奠定基础。方法采用ELISA、荧光定量RT-PCR、RT-PCR 3种方法对EV71病毒特异性和灵敏度进行综合分析。结果通过对比分析,ELISA方法检出阳性18份,阳性率为11.32%;实时荧光定量PCR方法检出阳性53份,阳性率为33.33%;RT-PCR方法检出阳性51份,阳性率为32.08%。结论 3种方法中实时荧光定量PCR方法具有较高的灵敏度及特异性,更具优势,更能满足临床需要。Objective To explore the different ways in enterovirus 71 （EV71） detection results compared,lay the foundation of quick diagnosis on the disease. Methods The three methods for ELISA, fluorescence quantitative RT - PCR and RT- PCR were used and compared for specificity and sensitivity of EV71. Results Through comparative analysis, eight- een samples were detected positive by ELISA methods, the positive rate was 11.32%. Fifty - three samples were tested positive by the fluorescence method for real - time RT - PCR , the positive rate was 33.33%. Fifty - one samples were detected positive by RT - PCR, the positive rate was 32.8%. Conclusion Fluorescent RT - PCR detection method has the performance of high sensitivity and specificity in the three methods, it can meet with the clinical needs better.

关 键 词：肠道病毒EV71 ELISA 实时荧光定量RT—PCR RT—PCR 

分 类 号：R512.5[医药卫生—内科学]