猪ESR基因一个外显子片段的克隆与序列分析  

CLONING AND SEQUENCE ANALYSIS OF THE DNA FRAGMENT OF A EXON OF THE PIG ESE GENE

作　　者：徐子清[1] 梅书棋[1] 樊俊华[1] 

机构地区：[1]湖北省农科院畜牧兽医研究所,武汉430209

出　　处：《畜牧兽医学报》2001年第2期101-107,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：参考人、鸡、鼠的ESR基因序列 ,设计一对引物 ,采用PCR技术扩增出猪ESR基因一段DNA片段 ,将该片段克隆到pGEM TEasy质粒中 ,重组质粒用PCR扩增和酶切分析进行阳性克隆鉴定 ,然后测定核苷酸序列 ,并推导其氨基酸序列。将测定的猪ESR基因的部分序列 (332bp)与人、鼠、鸡的序列进行比较 ,结果表明 :猪的核苷酸和氨基酸序列与人、鼠、鸡相比 ,同源性分别为90 0 6 %和 86 36 % ,85 54%和 88 18% ,74 10 %和 71 82 % ,显示了强的保守性 ,猪的核苷酸序列与人、鼠、鸡相比存在着 4处特有变异 ,氨基酸序列aa2 5～ 30存在高变异区。WT5BZ]According to the data of the human,chicken and rat ESR sequence,a pair of primer is designed.One DNA fragment of the pig ESR gene was amplified successfully by PCR,and was cloned into pGEM T Easy vector.The fragment was further identified by PCR,restriction enzymes analysis and sequence analysis.The peptide sequence of this fragment was deduced.The comparison of the fragment sequence with those of human,rat and chicken ESR gene shows:The homologies of the nucleotide sequence and peptide sequence of the fragment with human,rat and chicken’s are 90 06% and 86 36%,85 54% and 88 18%,74 10% and 71 82%,respectively,which indicates the ESR gene is highly conserved.The pig nucleotide sequence exits 4 special variation and the peptide sequence in amino acid 25～30 is poorly conserved. [WT5HZ]

关 键 词：猪 ESR基因 PCR扩增 序列分析 基因克隆 

分 类 号：S828.2[农业科学—畜牧学] S813.1[农业科学—畜牧兽医]