应用浸种法导入外源DNA转化烤烟遗传性状变异初探(Ⅱ)  

A Preliminary Study by Using Seed Soaking Method of Introduce Exogenous DNA to Induce the Genetic Characters in Flue cured Tobacco(Ⅱ)

作　　者：朱生伟[1] 徐仲[2] 张寒霜[3] 史芝文[2] 

机构地区：[1]中国科学院植物所,北京100093 [2]东北农业大学生物工程学院,黑龙江哈尔滨150030 [3]河北省农林科学院棉花所,河北石家庄050031

出　　处：《华北农学报》2001年第1期73-77,共5页Acta Agriculturae Boreali-Sinica

摘　　要：对应用浸种处理获得的变异后代 ,进行了叶绿素、糖、蛋白质、维生素C及株高、叶面积、生育期等的测定。试验表明 :该方法对株高、叶面积、生育期等性状的影响较为显著 ,对叶绿素、糖。In this experiment,the variant materials obtained by seed soaking method were tested for biochemical indices such as chlorophyll,soluble sugar,reducing sugar,protein and Vitamin C contents,as well as plant height,plant type,leaf area and growth period.The results showed that plant height,plant type,leaf area,growth period and disease resistance were significantly affected. However,the contents of chlorophyll,soluble sugar ,reducing sugar,protein and Vitamin C were not.The method has the advantages of high ratio of variation(21.2%),easy to operate,and the separations stablize quickly.

关 键 词：烤烟 浸种法 外源DNA导入 变异 遗传转化 

分 类 号：S572.03[农业科学—烟草工业]