肌苷氯化钠注射液中肌苷含量的两种测定方法比较  

Determination of Inosine in Inosine and Sodium Chloride Injections Respectively by UV Spectrophotometry and HPLC

作　　者：刘栋[1] 

机构地区：[1]解放军第153中心医院药械科,郑州450042

出　　处：《中国药师》2014年第6期964-966,共3页China Pharmacist

摘　　要：目的：比较肌苷氯化钠注射液中肌苷含量的两种测定方法.方法：分别采用高效液相色谱（HPLC）法及紫外分光光度（UV）法测定.HPLC法采用DiamonsilTM C18柱（250 mm×4.6 mm,5μm）,以甲醇-水（10∶ 90）为流动相,检测波长为248nm,流速为1.0 ml·min-1,进样量为20 μl,柱温为30℃.UV法采用水为溶剂,检测波长为248 nm.结果：HPLC法肌苷质量浓度在6.51 ～32.56 μg· ml-1范围内与峰面积线性关系良好,r=0.999 9,平均回收率为99.78％,RSD=0.24％（n=9）;UV法肌苷质量浓度在6.0～14.0 μg·ml-1范围内与吸光度线性关系良好,r =0.999 6,平均回收率为99.31％,RSD =0.25％ （n=9）.统计学结果显示两种方法测定结果差异无统计学意义（P＞0.05）.结论：UV法可以作为肌苷氯化钠注射液半成品含量的测定方法.Objective：To compare UV and HPLC method for the content determination of inosine in inosine and sodium injections.Methods：The content determination of inosine was determined by HPLC and UV method,respectively.The HPLC method adopted a DiamonsilTM C18 column（250 mm × 4.6 mm,5 μm） with the mobile phase of methanol-water（10∶ 90） and the detection wavelength of 248 nm.The flow rate was 1.0 ml · min-1,the injection volume was 20μ1 and the column temperature was 30℃.The UV method adopted the detection wavelength of 248 nm with water as the solvent.Results：There was a good linearity within the range of 6.51-32.56 μg · ml-1 for inosine （r =0.999 9） by HPLC.The average recovery was 99.78 % （RSD =0.24%,n =9）.There was a good linearity within the range of 6.0-14.0 μg · ml-1 for inosine （r =0.999 6） by UV.The average recovery was 99.31% （RSD =0.25 %,n =9）.There was no significant difference between the determination results of the two methods （P 〉 0.05）.Conclusion：The UV method can be used in the content determination of inosine in half-finished products of inosine and sodium injections.

关 键 词：肌苷 注射液 高效液相色谱法 紫外分光光度法 

分 类 号：R927.2[医药卫生—药学]