悬浮红细胞上清中与肿瘤相关非细胞成分蓄积规律研究  

Accumulative Regularity of Tumor-associated Noncellular Components in Supernatant of Stored Packed Red Cells

作　　者：庄远[1] 魏超[1] 张婷[1] 潘纪春[1] 汪德清[1] 

机构地区：[1]中国人民解放军总医院输血科,北京100853

出　　处：《中国实验血液学杂志》2014年第3期812-816,共5页Journal of Experimental Hematology

基　　金：2010年卫生部行业专项临床有效输血及血液风险控制技术应用研究与推广--子课题"红细胞输注与保存技术的应用研究与推广"(201002005)

摘　　要：本研究探讨悬浮红细胞(pRBC)上清中与肿瘤相关的非细胞成分在整个保存期中的蓄积规律。在采集当日对悬浮红细胞进行五联袋分装,于保存第0、7、14、21、28、和35 d各取30 ml红细胞悬液,以1006×g离心10 min后取上清,采用ELISA方法测定正常T细胞表达和分泌因子(RANTES/CCL5)、肿瘤坏死因子α(TNF-α)、血小板衍生生长因子(PDGF)、血管内皮生长因子(VEGF)和单核细胞趋化蛋白-1(MCP-1)蓄积浓度。结果表明:RANTES/CCL5和TNF-α在新鲜pRBC上清中保持有较高的浓度,随着保存期的延长,其蓄积浓度未见有明显增高。VEGF在保存当天的pRBC上清中浓度为229.9 pg/ml,保存7 d末浓度迅速上升至749.08 pg/ml,之后较稳定地维持在高水平,保存35 d末其浓度为760.67 pg/ml,其浓度曲线显示了保存7 d后的一个明显上升,但配对t检验未见有统计学差异。PDGF的蓄积规律表现为:PDGF在保存当天的pRBC上清中浓度为13.54 ng/L,保存期内稳定上升,28 d末略有下降,保存35 d末其浓度迅速上升为22.13 ng/L(P<0.05),显示浓度增长有统计学差异。MCP-1在保存当天的浓度为39.98 ng/L,保存期内缓慢上升,35 d末浓度为49.83 ng/L。结论:随着保存期的延长,pRBC上清中生长因子类物质似乎呈现明显的增长趋势,而RANTES/CCL5和TNF-α在新鲜pRBC上清中即保持有较高的浓度,且随着保存时间的延长蓄积浓度未见有明显增高,看来同保存期的延长无明显相关。This study was purposed to investigate the accumulative regularity of tumor-associated noncellular components in supernatant of stored packed red blood cells （PRBC） during storage. The supernatant of PRBC was obtained by centrifugation with 1 006 x g for 10 min at day 0, 7, 14, 21, 28 and 35 d. The enzyme-linked immunosorbent assay （ELISA） was used to detect the expression of T cells and the accumulative levels of secreted RANTES/CCL5, tumor mecrosis factor-alpha （TNF-α）, platelet derived growth factor （PDGF）, vascular endothelial growth factor （VEGF） and monocyte chemotactic protein-1 （MCP-1）. The results showed that the high concentration of RANTES/CCL5 and TNF-α was found in fresh PRBC, and their accumulative concentration did not increase along with the prolonging of storage time. The VEGF level in PRBC at day 0 of storage was 229.9 pg/ml, and increased to 749.08 pg/ml at end of day 7, then it was stable, and increased to 760.67 pg/ml at end of day 35. The PDGF level in surpernatant of PRBC was 13.54 ng/L at dag 0 of storage, and increased stably during storage, then decreased at day 28, however PDGF rapidly rose to 22.13 ng/L at the end of day 35 （ P 〈 0.05 ）. The MCP-1 level in supernatant of PRBC was 39.98 ng/L at day 0 of storage, then slowly increased during strorage time, at end of day 35 MCP-1 level increased to 49.83 ng/L. It is concluded that along with the prolongation of storage time, the growth factors in the supernatant of PRBC display the tendency of accumulative increment and RANTES/CCL5 and TNF-α show relative high level at day 0 of storage, moreover, no obvious increase of accumulation is observed along with prolonging of the storage time, suggesting no relation of concentration with storage time.

关 键 词：悬浮红细胞 非细胞成分物质 血小板衍生生长因子 血管内皮生长因子 肿瘤 

分 类 号：R331.141[医药卫生—人体生理学]