丙氨酸溶液作为A→O血型转变酶解缓冲液的研究  

Alanine Solution as Enzyme Reaction Buffer Used in A to O Blood Group Conversion

作　　者：李素波[1] 张雪[1] 张印则[2] 檀英霞[1] 鲍国强[1] 王颖丽[1] 季守平[1] 宫锋[1] 高红伟[1] 

机构地区：[1]军事医学科学院野战输血研究所血液分子生物学研究室,北京100850 [2]深圳市血液中心输血医学研究所,广东深圳518035

出　　处：《中国实验血液学杂志》2014年第3期817-820,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号:81300447)

摘　　要：本研究旨在探讨丙氨酸缓冲液对α-N-乙酰半乳糖胺酶发挥高效酶解A抗原活性的影响。用Western blot检测α-N-乙酰半乳糖胺酶在丙氨酸等不同缓冲液中与红细胞的结合能力。结果表明,丙氨酸溶液也可以作为A→O血型转变的酶解缓冲液,α-N-乙酰半乳糖胺酶在丙氨酸溶液中酶解A型红细胞所用剂量与在甘氨酸溶液中的相当;Western blot证实,在丙氨酸溶液中α-N-乙酰半乳糖胺酶与红细胞结合能力与在甘氨酸中相似,这也进一步证明α-N-乙酰半乳糖胺酶与红细胞的结合是α-N-乙酰半乳糖胺酶发挥酶解A抗原的活性所必需的。结论:丙氨酸溶液可以作为A→O血型转变的酶解缓冲液,在这一缓冲液中α-N-乙酰半乳糖胺酶与红细胞紧密结合,可以发挥高效的酶解A抗原的活性。The aim of this study was to investigate the effect of alanine solution as α-N-acetylgalactosaminidase enzyme reaction buffer on the enzymatic activity of A antigen. The binding ability of α-N-acetylgalactosarninidase with RBC in different reaction buffer such as alanine solution, glycine solution, normal saline （0.9% NaC1）, PBS, PCS was detected by Western blot. The results showed that the efficiency of A to O conversion in alanine solution was similar to that in glycine solution, and Western blot confimed that most of enzymes binded with RBC in glycine or aianine solution, but few enzymes binded with RBC in PBS, PCS or normal saline. The evidences indicated that binding of enzyme with RBC was a key element for A to O blood group conversion, while the binding ability of α-N-acetylgalactosaminidase with RBC in alanine or glycine solution was similar. It is concluded that alanine solution can be used as enzyme reaction buffer in A to O blood group conversion. In this buffer, the α-N- closely binded with RBC and α-N-acetylgalactosaminidase plays efficient enzymatic activity of A antigen.

关 键 词：α-N-乙酰半乳糖胺酶 血型转变 丙氨酸 通用型红细胞 

分 类 号：R457.11[医药卫生—治疗学]