宁夏人群载脂蛋白E基因多态性与血清尿酸水平的相关性研究  

Correlation of ApoE gene polymorphism and serum uric acid in Ningxia Hui Autonomous Region

作　　者：吴洁[1] 邱玲[1] 程歆琦[1] 王青[2] 倪兰[2] 国秀芝[1] 程倩[1] 韩建华[1] 徐涛[3] 朱广瑾[3] 

机构地区：[1]中国医学科学院北京协和医院检验科,100730 [2]宁夏医科大学总医院医学实验中心 [3]中国医学科学院基础医学研究所

出　　处：《中华检验医学杂志》2014年第6期434-438,共5页Chinese Journal of Laboratory Medicine

基　　金：国家科技部重点专科建设项目资助（2006FY110300）

摘　　要：目的：探讨我国宁夏人群中载脂蛋白E（ ApoE）基因多态性与血清中尿酸水平的相关性。方法病例对照研究。2011年10月至11月，随机选取宁夏地区528名健康体检人群（男296名、女232名），采集空腹静脉血进行血生化检测，应用等位基因特异性多重PCR（multi-ARMS PCR）技术，对ApoE基因进行分型，分析基因型与血清血脂水平和尿酸水平的关系。非正态分布者比较采用致和检验。结果 ApoE常见的6种基因型均可检出，不同ApoE基因型的总胆固醇（ TC）、低密度脂蛋白胆固醇（ LDL-C ）和尿酸（ UA ）水平差异有统计学意义。 ApoE ε2/3基因型的 TC [（3.50±0.83）mmol/L]、LDL-C[（1.87±0.60） mmol/L]水平低于ε3/3[（3.85±0.84）mmol/L、（2.24±0.69） mmol/L]和ε3/4[（3.89±0.78） mmol/L、（2.34±0.68） mmol/L]基因型，差异有统计学意义，F＝5.619，P＜0.05。 UA水平[（374.9±97.8） mmol/L]则显著高于ε3/3[（328.4±105.5） mmol/L]和ε3/4[（339.2±98.3） mmol/L]，差异有统计学意义，F＝6.052，P＜0.05。进一步对年龄、性别、地区等因素进行校正后ApoE基因多态性对UA水平的影响依然显著。结论 ApoE基因多态性与血清尿酸水平相关，含ε2等位基因者具有更高的血清尿酸水平。（中华检验医学杂志，2014，37：434-438）Objective Toexplore the correlation of ApoE gene polymorphism and serum uric acid levels in Ningxia Hui Autonomous Region , China.Methods A case-control study.October 2011 to November 2011, five hundred twenty eight （ 296 male, 232 female ） apparently healthy individuals were studied.Questionnaires and physical examinations were performed by standard operation procedure.Fasting blood was collected for biochemistry testing including serum lipid parameters , uric acid concentration and creatinine levels.The multi-ARMS PCR was applied to determine ApoE genotypes ,and the relation of ApoE genotypes with serum lipid parameters and uric acid levels were analyzed.Non-normal distribution were compared using cause and inspection.Results The common six kinds of ApoE genotype can be detected.The total cholesterol （ TC） ,low density lipoprotein cholesterol （ LDL-C） and uric acid （ UA） levels in different genotype subgroups had statistical differences.The individuals with ε2/3 genotype had a significantly greater reductions in TC and LDL-C levels but increment in uric acid concentration than those withε3/3 and ε3/4 genotype （P〈0.05）.The effect of ApoE gene polymorphism on uric acid levels still remained significantly after adjustment for age , gender , region and other factors.Conclusion The ApoE polymorphism is associated with serum uric acid levels and individuals with ε2 allele have higher serum uric acid levels.

关 键 词：载脂蛋白E类 多态现象 遗传 尿酸 脂类代谢 

分 类 号：R587.2[医药卫生—内分泌]