梓醇对糖尿病大鼠主动脉的保护作用与抗氧化机制研究  

Protective effect of catalpol on diabetic rat aorta and its antioxidant mechanisms

作　　者：刘江月[1] 

机构地区：[1]潍坊医学院病理生理教研室,山东潍坊261053

出　　处：《中国病理生理杂志》2014年第6期1023-1028,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.8130068);山东省自然科学基金资助项目(No.ZR2009CQ013);山东省中医药管理局资助项目(No.2013-237)

摘　　要：目的:研究梓醇对Goto-Kakizaki(GK)大鼠主动脉的保护作用并探讨其抗氧化机制。方法:6月龄GK大鼠45只随机分为糖尿病模型组、二甲双胍(100 mg·kg-1·d-1)组以及梓醇高剂量(100 mg·kg-1·d-1)组、中剂量(50 mg·kg-1·d-1)组、低剂量(10 mg·kg-1·d-1)组,10只雄性Wistar大鼠作为对照组。各组灌胃给药12周,对照组和模型组给予等量的生理盐水。检测大鼠空腹血糖(FBG)和血脂;检测血清活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化力(T-AOC)水平;Western blotting检测胸主动脉组织核因子E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)表达;HE染色观察胸主动脉病理变化;透射电镜观察胸主动脉组织超微结构变化。结果:梓醇治疗后,血糖和血脂显著下降;血清ROS和MDA水平明显降低,SOD活性和T-AOC显著增强;胸主动脉组织Nrf2和HO-1蛋白表达明显增强;HE染色显示胸主动脉病变程度明显减轻,透射电镜观察胸主动脉超微结构损伤明显减轻。结论:梓醇能够有效保护GK大鼠胸主动脉,其机制可能与降低血糖和血脂,减轻氧化应激反应,激活Nrf2/ARE/HO-1信号通路有关。AIM：To investigate the protective effect of catalpol on Goto-kakizaki （GK） rat aorta and to ex-plore its antioxidant mechanisms.METHODS：Six-month-old GK rats （n=45） were randomly divided into diabetic model group, metformin （100 mg ·kg^-1· d^-1） group, and high-dose （100 mg ·kg^-1· d^-1）, medium-dose （50 mg ·kg^-1· d^-1） and low-dose （10mg ·kg^-1· d^-1） catalpol groups.The healthy male Wistar rats （n=10） were used as control group.The rats in control and model groups were given a same volume of saline .All reagents were administered by oral gavage for 12 weeks.Blood glucose and lipids were detected by an automatic biochemical analyzer .Serum reactive oxygen species （ROS）, malondialdehyde （MDA）, superoxide dismutase （SOD） and total antioxidant capacity （T-AOC） levels were detected by commercial kits .The expression of nuclear factor E2-related factor 2 （Nrf2） and heme oxygenase-1 （HO-1） in the thoracic aorta was determined by Western blotting .The pathological changes of the thoracic aorta were observed by HE staining.The ultrastructural changes of the thoracic aorta were observed under electron microscope .RESULTS：Af-ter catalpol treatment , the levels of blood glucose and blood lipids were decreased significantly , and serum levels of ROS and MDA were significantly decreased , but the activity of SOD and T-AOC were significantly enhanced .The protein expression of Nrf2 and HO-1 in the thoracic aorta were significantly increased , the thoracic aortic lesions indicated by HE staining significantly reduced , and the thoracic aortic damage under ultrastructural observation was attenuated slightly . CONCLUSION：Catalpol effectively protects GK rat thoracic aorta , which may be associated with decreasing blood lipids , reducing oxidative stress and activating Nrf 2/ARE/HO-1 signaling pathways.

关 键 词：Goto-Kakizaki大鼠 糖尿病 2型 梓醇 氧化性应激 

分 类 号：R543.1[医药卫生—心血管疾病]